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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MK Polyclonal Antibody
- 货号:YT5177
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat
- 免疫原:
- The antiserum was produced against synthesized peptide derived from the Internal region of human MDK. AA range:94-143
- 特异性:
- MK Polyclonal Antibody detects endogenous levels of MK protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MDK;MK1;NEGF2;Midkine;MK;Amphiregulin-associated protein;ARAP;Midgestation and kidney protein;Neurite outgrowth-promoting factor 2;Neurite outgrowth-promoting protein
- 背景:
- This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012],
- 功能:
- function:Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.,induction:By heparin and retinoic acid.,similarity:Belongs to the pleiotrophin family.,subunit:Homodimer.,
- 组织表达:
- Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions.
- Western Blot analysis of SW480, MCF7, PC-3, Raji, K562, mouse eye, mouse kidney cells using MK Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
- Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
- Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
- Western blot analysis of lysate from SW480 cells, using MDK Antibody.