GAPDH Monoclonal Antibody(2B8)

    • 货号:YM3029
    • 应用:WB,IF/ICC,IHC-p
    • 种属:Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Pig,sheep,Insect,Yeast
      • 基因名称:
      • GAPDH
      • 蛋白名称:
      • Glyceraldehyde-3-phosphate dehydrogenase
      • Human Gene Id:
      • 2597
      • Human Swiss Prot No:
      • P04406
      • Mouse Swiss Prot No:
      • P16858
      • 免疫原:
      • Synthetic Peptide of GAPDH
      • 特异性:
      • The antibody detects endogenous GAPDH protein.
      • 组成:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • 来源:
      • Mouse
      • 稀释:
      • WB: 1:5000-20000 IHC: 1:200-300 IF 1:200
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
      • 实测条带:
      • 37
      • 背景:
      • glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
      • 功能:
      • catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase fami
      • 细胞定位:
      • nucleus,cytoplasm,lipid particle,cytosol,plasma membrane,microtubule cytoskeleton,membrane,intracellular ribonucleoprotein complex,extracellular matrix,nuclear membrane,vesicle,intracellular membrane-bounded orga
      • 组织表达:
      • Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu

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      Wu, Shiyong, et al. "Trimetazidine protects against myocardial ischemia/reperfusion injury by inhibiting excessive autophagy." Journal of Molecular Medicine 96.8 (2018): 791-806.
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      Li, Gang, et al. "SOST, an LNGFR target, inhibits the osteogenic differentiation of rat ectomesenchymal stem cells." Cell proliferation 51.2 (2018): e12412.
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      Zhi, Hui, et al. "Decabromodiphenyl ether (BDE-209) enhances foam cell formation in human macrophages via augmenting Toll-like receptor 4-dependent lipid uptake." Food and Chemical Toxicology 121 (2018): 367-373.
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      Liu, Wei-hua, et al. "Low-dose decitabine induces MAGE-A expression and inhibits invasion via suppression of NF-κB2 and MMP2 in Eca109 cells." Biomedicine & Pharmacotherapy 68.6 (2014): 745-750.
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      Yang, Deng-Hua, et al. "The histone deacetylase inhibitor sodium butyrate protects against noise-induced hearing loss in Guinea pigs." Neuroscience letters 660 (2017): 140-146.
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      Wei, Yuzhen, et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.
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      Liu, Xuemei, et al. "improvement in cerebral ischemia–reperfusion injury through the Tlr4/nf-κb pathway after Kudiezi injection in rats." Life sciences 191 (2017): 132-140.
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      Wang, Yan, et al. "Suppressive effect of β, β-dimethylacryloyl alkannin on activated dendritic cells in psoriasis by the TLR7/8 pathway." International immunopharmacology 40 (2016): 410-418.
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      Gong, Ningning, et al. "Adipokine expression and endothelial function in subclinical hypothyroidism rats." Endocrine connections 7.2 (2018): 295-304.
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      Zhou, Xi-Dan, Dong-Dong Shi, and Zhang-Jin Zhang. "Antidepressant and anxiolytic effects of the proprietary Chinese medicine Shexiang Baoxin pill in mice with chronic unpredictable mild stress." Journal of food and drug analysis(2018).
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      Wang, Zeming, et al. "Effects of icariin on the regulation of the OPG‑RANKL‑RANK system are mediated through the MAPK pathways in IL‑1β‑stimulated human SW1353 chondrosarcoma cells." International journal of molecular medicine 34.6 (2014): 1720-1726.
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      Zhang, Chengke, et al. "Induction of size-dependent breakdown of blood-milk barrier in lactating mice by TiO2 nanoparticles." PloS one 10.4 (2015): e0122591.
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      Liang, Xiaofang, et al. "Growth and feed intake regulation responses to anorexia, adaptation and fasting in Japanese seabss, Lateolabrax japonicas when fishmeal is totally replaced by plant protein." Aquaculture 498 (2019): 528-538.
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      Du, Shaobo, et al. "Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells against Ultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation." BioMed research international 2017 (2017).
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      Jing, Shen, et al. "Imaging Potential Evaluation of Fab Derived from the Anti-EGFRvIII Monoclonal Antibody 4G1." Radiation research (2018).
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      Zeng, Jin, et al. "Regulation of intrinsic apoptosis in cycloheximide-treated macrophages by the Sichuan human strain of Chinese Leishmania isolates." Acta tropica 153 (2016): 101-110.
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      Zhang, Lei, et al. "Increased α-actinin-2 expression in the atrial myocardium of patients with atrial fibrillation related to rheumatic heart disease." Cardiology 135.3 (2016): 151-159.
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      Chen, Luchao, et al. "Effects of purified Omphalia lapidescens protein on metastasis, cell cycle, apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.
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      Jia, Yan-Fei, et al. "Expression of kisspeptin-GnRH system is down-regulated in hypothalamic arcuate nucleus of male rats with high-fat-diet." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10.5 (2017): 6099-6107.
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      谢万生, et al. "3 种 MAPK 抑制因子对亚砷酸钠活化 LX-2 细胞 TGF-β1 和 α-SMA 的影响." 中华地方病杂志 38.2: 96-100.
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      Xiao, Bin, et al. "Beta-asarone alleviates myocardial ischemia-reperfusion injury by inhibiting inflammatory response and NLRP3 inflammasome mediated pyroptosis." Biological and Pharmaceutical Bulletin (2020): b19-00926.
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      Cheng, Xiaocheng, et al. "TNAP is a novel regulator of cardiac fibrosis after myocardial infarction by mediating TGF-β/Smads and ERK1/2 signaling pathways." EBioMedicine 67 (2021): 103370.
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      Wei, Yuzhen, et al. "Interleukin‐38 alleviates cardiac remodelling after myocardial infarction." Journal of cellular and molecular medicine 24.1 (2020): 371-384.
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      Wang, Yingying, et al. "p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathway." Cell proliferation 53.4 (2020): e12800.
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      Zhao, Manzhu, et al. "The role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells." Cell proliferation53.2 (2020): e12758.
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      Ho, Philip Wing-Lok, et al. "Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA)." Autophagy 16.2 (2020): 347-370.
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      Yi, Huan, et al. "Downregulation of RIPK4 Expression Inhibits Epithelial-Mesenchymal Transition in Ovarian Cancer through IL-6." Journal of Immunology Research 2021 (2021).
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      Li, Yafei, et al. "Resveratrol prevents ISO-induced myocardial remodeling associated with regulating polarization of macrophages through VEGF-B/AMPK/NF-kB pathway." International immunopharmacology 84 (2020): 106508.
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      Sun, Qin-Juan, et al. "The Role of bone morphogenetic protein 9 in nonalcoholic fatty liver disease in mice." Frontiers in Pharmacology 11 (2020).
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      Fu, Chen, et al. "Neuroprotective effects of Qingnao dripping pills against cerebral ischemia via inhibiting NLRP3 Inflammasome signaling pathway: in vivo and in vitro." Frontiers in pharmacology 11 (2020): 65.
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      Zhu, Maoshu, et al. "miR‑142‑5p promotes renal cell tumorigenesis by targeting TFAP2B." Oncology Letters 20.6 (2020): 1-1.
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      Zhu, Xiaodan, et al. "WD-3 inhibits the proliferation of breast cancer cells by regulating the glycolytic pathway." Bosnian journal of basic medical sciences 20.2 (2020): 226.
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      Zheng, Jing-min, et al. "KDF1, a novel tumor suppressor in clear cell renal cell carcinoma." Frontiers in Oncology 11 (2021).
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      • 产品图片
      • Cheng, Xiaocheng, et al. "TNAP is a novel regulator of cardiac fibrosis after myocardial infarction by mediating TGF-β/Smads and ERK1/2 signaling pathways." EBioMedicine 67 (2021): 103370.
      • Wang, Yingying, et al. "p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathway." Cell proliferation 53.4 (2020): e12800.
      • Ho, Philip Wing-Lok, et al. "Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA)." Autophagy 16.2 (2020): 347-370.
      • Wei, Yuzhen, et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.
      • Zhang, Chengke, et al. "Induction of size-dependent breakdown of blood-milk barrier in lactating mice by TiO2 nanoparticles." PloS one 10.4 (2015): e0122591.
      • Du, Shaobo, et al. "Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells against Ultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation." BioMed research international 2017 (2017).
      • Chen, Luchao, et al. "Effects of purified Omphalia lapidescens protein on metastasis, cell cycle, apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.
      • Immunofluorescence analysis of Hela cell. 1,Cyclin D1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GAPDH Monoclonal Antibody(2B8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.
      • Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,GAPDH Monoclonal Antibody(2B8) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunofluorescence analysis of Human-colon tissue. 1,GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of Mouse-liver tissue. 1,GAPDH Monoclonal Antibody(2B8)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle (3), Sheep Muscle (4), and Mouse brain (5), diluted at 1:10000.
      • The picture was kindly provided by our customer
      • The picture was kindly provided by our customer
      • The picture was kindly provided by our customer