β-actin Monoclonal Antibody(5B7)

    • 货号:YM3028
    • 应用:IF/ICC,WB,IHC-p
    • 种属:Human,Rat,Mouse,Mk,Dg,Ch,Hamster,Rb,Insect
      • 基因名称:
      • ACTB
      • 蛋白名称:
      • Actin cytoplasmic 1
      • Human Gene Id:
      • 60
      • Human Swiss Prot No:
      • P60709
      • Mouse Swiss Prot No:
      • P60710
      • 免疫原:
      • Synthetic Peptide of β-actin
      • 特异性:
      • The antibody detects endogenous β-actin protein.
      • 组成:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • 来源:
      • Mouse
      • 稀释:
      • IF: 1:50-200 WB: 1:5000-20000 IHC: 1:200-300
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
      • 分子量:
      • 41737
      • 实测条带:
      • 43
      • 背景:
      • actin beta(ACTB) Homo sapiens This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the
      • 细胞定位:
      • nuclear chromatin,extracellular space,nucleoplasm,cytoplasm,cytosol,cytoskeleton,plasma membrane,focal adhesion,membrane,intracellular ribonucleoprotein complex,cortical cytoskeleton,NuA4 histone acetyltransferase complex,cyt
      • 组织表达:
      • B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car

      Yan, Shi-Hai, et al. "Modulations of Keap1-Nrf2 signaling axis by TIIA ameliorated the oxidative stress-induced myocardial apoptosis." Free Radical Biology and Medicine 115 (2018): 191-201.
      货号:ym3028

      Lin, Dandan, et al. "MXRA7 deficiency alleviates carbon tetrachloride-induced acute liver injury in mice." Frontiers in immunology 9 (2018): 773.
      货号:ym3028

      Bai, Jingchao, et al. "HSP 90 inhibitor AUY 922 can reverse Fulvestrant induced feedback reaction in human breast cancer cells." Cancer science 108.6 (2017): 1177-1184.
      货号:ym3028

      Tang, Qiusha, et al. "Combination of PEI-Mn0. 5Zn0. 5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma." International journal of nanomedicine 10 (2015): 7129.
      货号:ym3028

      Wang, Wu M., et al. "MicroRNA-182 regulates neurite outgrowth involving the PTEN/AKT pathway." Frontiers in cellular neuroscience 11 (2017): 96.
      货号:YM3028

      Wang, Jianping, et al. "Vanadate oxidative and apoptotic effects are mediated by the MAPK-Nrf2 pathway in layer oviduct magnum epithelial cells." Metallomics 9.11 (2017): 1562-1575.
      货号:ym3028

      Liu, Chang, et al. "Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α." Stem cells international 2018 (2018).
      货号:ym3028

      Yu, Qianqian, et al. "Unraveling proteome changes of Holstein beef M. semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry." Journal of proteomics 154 (2017): 85-93.
      货号:YM3028

      Cheng, Yahui, et al. "The role of PTEN in regulation of hepatic macrophages activation and function in progression and reversal of liver fibrosis." Toxicology and applied pharmacology 317 (2017): 51-62.
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      Yuanfeng, Wu, et al. "Dietary sulforaphane inhibits histone deacetylase activity in B16 melanoma cells." Journal of Functional Foods 18 (2015): 182-189.
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      Lan, Rui, et al. "Mitophagy is activated in brain damage induced by cerebral ischemia and reperfusion via the PINK1/Parkin/p62 signalling pathway." Brain research bulletin142 (2018): 63-77.
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      Liu, Yanmei, et al. "Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour." Journal of Cancer 9.8 (2018): 1486.
      货号:ym3028

      Tao, Yuquan, et al. "Huaier Augmented the Chemotherapeutic Sensitivity of Oxaliplatin via Downregulation of YAP in Hepatocellular Carcinoma." Journal of Cancer 9.21 (2018): 3962.
      货号:YM3028

      Zhang, Lai-Bo, et al. "Calcitonin protects chondrocytes from lipopolysaccharide-induced apoptosis and inflammatory response through MAPK/Wnt/NF-κB pathways." Molecular immunology 87 (2017): 249-257.
      货号:ym3028

      Zou, Mingyue, et al. "Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer." Oncology reports 41.2 (2019): 989-998.
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      Xu, Yingming, et al. "The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain." PloS one 13.7 (2018): e0201021.
      货号:ym3028

      Ma, Kun, et al. "Neurotoxicity effects of atrazine-induced SH-SY5Y human dopaminergic neuroblastoma cells via microglial activation." Molecular BioSystems 11.11 (2015): 2915-2924.
      货号:ym3028

      Zhou, Jianyu, et al. "Facilitating colorectal cancer cell metastasis by targeted binding of long non-coding RNA ENSG00000231881 with miR-133b via VEGFC signaling pathway." Biochemical and biophysical research communications 509.1 (2019): 1-7.
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      Zeng, Jin, et al. "Regulation of intrinsic apoptosis in cycloheximide-treated macrophages by the Sichuan human strain of Chinese Leishmania isolates." Acta tropica 153 (2016): 101-110.
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      Wu, Shouli, et al. "Aberrant methylation‐induced dysfunction of p16 is associated with osteoblast activation caused by fluoride." Environmental toxicology 34.1 (2019): 37-47.
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      Wang, Jun-Jie, et al. "Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment." Neural regeneration research 14.3 (2019): 452.
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      Wang, Jun-ying, et al. "Repeated electroacupuncture treatment attenuated hyperalgesia through suppression of spinal glial activation in chronic neuropathic pain rats." BMC complementary and alternative medicine 18.1 (2018): 74.
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      Chen, Xi, et al. "Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6." Molecular medicine reports 12.4 (2015): 5155-5162.
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      Wu, Aibing, et al. "Upregulation of microRNA‑492 induced by epigenetic drug treatment inhibits the malignant phenotype of clear cell renal cell carcinoma in vitro." Molecular medicine reports 12.1 (2015): 1413-1420.
      货号:YM3028

      Wu, Aibing, et al. "Upregulation of microRNA‑492 induced by epigenetic drug treatment inhibits the malignant phenotype of clear cell renal cell carcinoma in vitro." Molecular medicine reports 12.1 (2015): 1413-1420.
      货号:YM3028

      Chen, Ruonan, et al. "The permeability characteristics and interaction of main components from Si-Ni-San in a MDCK epithelial cell monolayer model." Xenobiotica just-accepted (2017): 1-26.
      货号:ym3028

      Dong, Na, et al. "Ethyl pyruvate protects against Salmonella intestinal infection in mice through down-regulation of pro-inflammatory factors and inhibition of TLR4/MAPK pathway." International immunopharmacology 71 (2019): 155-163.
      货号:YM3028

      Song, Zonggong, et al. "miR-352 participates in the regulation of trypsinogen activation in pancreatic acinar cells by influencing the function of autophagic lysosomes." Oncotarget9.13 (2018): 10868.
      货号:YM3028

      Wen, Jiexia, et al. "Essential role of Na+/Ca2+ exchanger 1 in smoking-induced growth and migration of esophageal squamous cell carcinoma." Oncotarget 7.39 (2016): 63816.
      货号:ym3028

      Yang, Chun‐He, et al. "Melatonin promotes secondary hair follicle development of early post‐natal cashmere goat and improves cashmere quantity and quality by enhancing antioxidant capacity and suppressing apoptosis." Journal of pineal research (2019): e12569.
      货号:YM3028

      Zhang, Bo, Kun Ma, and Baixiang Li. "Inflammatory reaction regulated by microglia plays a role in atrazine-induced dopaminergic neuron degeneration in the substantia nigra." The Journal of toxicological sciences 40.4 (2015): 437-450.
      货号:ym3028

      Zhuang, Hao, et al. "Glycine decarboxylase induces autophagy and is downregulated by miRNA-30d-5p in hepatocellular carcinoma." Cell death & disease 10.3 (2019).
      货号:YM3028

      艾丽雅, et al. "蒙医不同针刺法对抑郁大鼠前额叶单胺类神经递质及 PKA/CREB/BDNF 通路的影响." 中国医药导报 16.8: 9-12.
      货号:YM3028

      • 产品图片
      • Immunofluorescence analysis of Hela cell. 1,Cyclin A Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.
      • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,β-actin Monoclonal Antibody(5B7) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western blot analysis of Hela (1) Rat brain (2) Mouse brain (3) Chicken lung (4) and Rabbit testis (5), diluted at 1:10000.
      • IHC Staining of Human ovary tissue, diluted at 1:200
      • Zhuang, Hao, et al. "Glycine decarboxylase induces autophagy and is downregulated by miRNA-30d-5p in hepatocellular carcinoma." Cell death & disease 10.3 (2019).
      • Bai, Jingchao, et al. "HSP 90 inhibitor AUY 922 can reverse Fulvestrant induced feedback reaction in human breast cancer cells." Cancer science 108.6 (2017): 1177-1184.
      • Tang, Qiusha, et al. "Combination of PEI-Mn0. 5Zn0. 5Fe2O4 nanoparticles and pHsp 70-HSV-TK/GCV with magnet-induced heating for treatment of hepatoma." International journal of nanomedicine 10 (2015): 7129.
      • Wang, Wu M., et al. "MicroRNA-182 regulates neurite outgrowth involving the PTEN/AKT pathway." Frontiers in cellular neuroscience 11 (2017): 96.
      • Liu, Chang, et al. "Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-α." Stem cells international 2018 (2018).
      • Tao, Yuquan, et al. "Huaier Augmented the Chemotherapeutic Sensitivity of Oxaliplatin via Downregulation of YAP in Hepatocellular Carcinoma." Journal of Cancer 9.21 (2018): 3962.
      • Liu, Yanmei, et al. "Cancer Stem Cells are Regulated by STAT3 Signalling in Wilms Tumour." Journal of Cancer 9.8 (2018): 1486.
      • Xu, Yingming, et al. "The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain." PloS one 13.7 (2018): e0201021.
      • Wang, Jun-Jie, et al. "Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment." Neural regeneration research 14.3 (2019): 452.
      • Wen, Jiexia, et al. "Essential role of Na+/Ca2+ exchanger 1 in smoking-induced growth and migration of esophageal squamous cell carcinoma." Oncotarget 7.39 (2016): 63816.
      • Song, Zonggong, et al. "miR-352 participates in the regulation of trypsinogen activation in pancreatic acinar cells by influencing the function of autophagic lysosomes." Oncotarget9.13 (2018): 10868.