ULK1 Polyclonal Antibody

    • 货号:YT4819
    • 应用:IHC;IF;WB;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • ULK1
      • 简介:
      • >>Mitophagy - animal;>>Autophagy - animal;>>mTOR signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases
      • 基因名称:
      • ULK1
      • 蛋白名称:
      • Serine/threonine-protein kinase ULK1
      • Human Gene Id:
      • 8408
      • Human Swiss Prot No:
      • O75385
      • Mouse Swiss Prot No:
      • O70405
      • 免疫原:
      • Synthesized peptide derived from the C-terminal region of human ULK1.
      • 特异性:
      • ULK1 Polyclonal Antibody detects endogenous levels of ULK1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000,IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ULK1;KIAA0722;Serine/threonine-protein kinase ULK1;Autophagy-related protein 1 homolog;ATG1;hATG1;Unc-51-like kinase 1
      • 分子量:
      • 113kD
      • 背景:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with GABARAP and GABARAPL2.,tissue specificity:Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.,
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with GABARAP and GABARAPL2.,tissue specificity:Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.,
      • 细胞定位:
      • Cytoplasm, cytosol . Preautophagosomal structure . Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. .
      • 组织表达:
      • Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

      Ferritinophagy is involved in Bisphenol A-induced ferroptosis of renal tubular epithelial cells through the activation of the AMPK-mTOR-ULK1 pathway Food Chem Toxicol. 2022 May;163:112909. WB Mouse kidney tissue
      货号:YT4819

      • 产品图片
      • Western Blot analysis of various cell lysis(provided by our customer). Primary Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS23920 was diluted at 1:10000
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).