p47-phox Polyclonal Antibody

    • 货号:YT3520
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • p47-phox
      • 简介:
      • >>Chemokine signaling pathway;>>Phagosome;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Prion disease;>>Leishmaniasis;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis
      • 基因名称:
      • NCF1
      • 蛋白名称:
      • Neutrophil cytosol factor 1
      • Human Swiss Prot No:
      • P14598
      • Mouse Swiss Prot No:
      • Q09014
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human p47 phox. AA range:341-390
      • 特异性:
      • p47-phox Polyclonal Antibody detects endogenous levels of p47-phox protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • NCF1;NOXO2;SH3PXD1A;Neutrophil cytosol factor 1;NCF-1;47 kDa autosomal chronic granulomatous disease protein;47 kDa neutrophil oxidase factor;NCF-47K;Neutrophil NADPH oxidase factor 1;Nox organizer 2;Nox-organizing protein 2;SH3
      • 实测条带:
      • 44kD
      • 背景:
      • The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.,function:NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).,online information:NCF1 deficiency database,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with NOXA1.,
      • 细胞定位:
      • Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side .
      • 组织表达:
      • Detected in peripheral blood monocytes and neutrophils (at protein level).

      Normothermic machine perfusion attenuates hepatic ischaemia-reperfusion injury by inhibiting CIRP-mediated oxidative stress and mitochondrial fission. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2021 Dec;25(24):11310-11321 WB Rat 1:1000 Liver
      货号:YT3520

      Change in Oxidative Stress and Mitochondrial Dynamics in Response to Elevated Cold-Inducible RNA-Binding Protein in Cardiac Surgery-Associated Acute Kidney Injury Oxidative Medicine and Cellular Longevity Yang Yan WB Rat
      货号:YT3520

      • 产品图片
      • Western Blot analysis of various cells using p47-phox Polyclonal Antibody
      • Western Blot analysis of COLO205 cells using p47-phox Polyclonal Antibody
      • Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using p47 phox Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HT-29, COLO205, and HepG2 cells, , using p47 phox Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from COLO205 cells using p47 phox antibody.