N-cadherin Polyclonal Antibody

    • 货号：YT2988
    • 应用：WB;IHC;IF;ELISA
    • 种属：Human;Mouse;Rat
      • 靶点：
      • N-cadherin
      • 简介：
      • >>Cell adhesion molecules;>>Arrhythmogenic right ventricular cardiomyopathy
      • 基因名称：
      • CDH2
      • 蛋白名称：
      • Cadherin-2
      • Human Gene Id：
      • 1000
      • Human Swiss Prot No：
      • P19022
      • Mouse Swiss Prot No：
      • P15116
      • 免疫原：
      • The antiserum was produced against synthesized peptide derived from human CDH2. AA range:721-770
      • 特异性：
      • N-cadherin Polyclonal Antibody detects endogenous levels of N-cadherin protein.
      • 组成：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源：
      • Polyclonal, Rabbit,IgG
      • 稀释：
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺：
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度：
      • 1 mg/ml
      • 储存：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称：
      • CDH2;CDHN;NCAD;Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD antigen CD325
      • 实测条带：
      • 100-140kD
      • 背景：
      • This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015],
      • 功能：
      • function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism.,similarity:Contains 5 cadherin domains.,subunit:Interacts with CDCP1.,
      • 细胞定位：
      • Cell membrane ; Single-pass type I membrane protein . Cell membrane, sarcolemma . Cell junction . Cell surface . Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. .
      • 组织表达：
      • Brain,Epithelium,Liver,

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      • 产品图片
      • Immunofluorescence analysis of rat-lung tissue. 1,N-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of rat-lung tissue. 1,N-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of rat-kidney tissue. 1,N-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of rat-kidney tissue. 1,N-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of mouse-kidney tissue. 1,N-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunofluorescence analysis of mouse-kidney tissue. 1,N-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
      • Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,N-cadherin Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
      • Western Blot analysis of various cells using N-cadherin Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of K562 cells using N-cadherin Polyclonal Antibody diluted at 1:1000
      • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Western blot analysis of lysates from COLO205 and K562 cells, using CDH2 Antibody. The lane on the right is blocked with the synthesized peptide.

      相关报价

      • 货号：
      • YT2988
      • 规格：
      • 40μL / 100μL / 200μL
      • 价格：
      • ¥960 / 2300 / 3780
      • 货期：
      • 预计发货时间为
        2024-04-30
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