• 靶点：
• MEK-1/2

• 简介：
• >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Cellular senescence;>>Vascular smooth muscle contraction;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>Gap junction;>>Signaling pathways regulating pluripotency of stem cells;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>Natural killer cell mediated cytotoxicity;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>Fc gamma R-mediated phagocytosis;>>TNF signaling pathway;>>Lon

• 基因名称：
• MAP2K1/MAP2K2

• 蛋白名称：
• Dual specificity mitogen-activated protein kinase kinase 1/2

• Human Gene Id：
• 5604/5605

• Human Swiss Prot No：
• Q02750/P36507

• Mouse Gene Id：
• 26395/26396

• Rat Gene Id：
• 170851/58960

• Rat Swiss Prot No：
• Q01986/P36506

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human MEK1/2. AA range:189-238

• 特异性：
• MEK-1/2 Polyclonal Antibody detects endogenous levels of MEK-1/2 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IF 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• MAP2K1;MEK1;PRKMK1;Dual specificity mitogen-activated protein kinase kinase 1;MAP kinase kinase 1;MAPKK 1;MKK1;ERK activator kinase 1;MAPK/ERK kinase 1;MEK 1;MAP2K2;MEK2;MKK2;PRKMK2;Dual specificity mitogen-activated protein k

• 实测条带：
• 43kD

• 背景：
• The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.,enzyme reg

• 细胞定位：
• Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body . Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein . Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742). .

• 组织表达：
• Widely expressed, with extremely low levels in brain.

Effects of hydroxysafflor yellow A on rats with collagen-induced arthritis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2021 Sep;570:26 WB Rat 1:500 Synovial
货号：YT2715

The MEK/ERK/CREB signaling pathway is involved in atrazine induced hippocampal neurotoxicity in Sprague Dawley rats. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2018 Dec 20 WB Rat 1:1500 hippocampal tissue
货号：YT2715

CPNE1 silencing inhibits the proliferation, invasion and migration of human osteosarcoma cells. ONCOLOGY REPORTS 2017 Dec 04 WB Human 1:1000 Saos-2 cell
货号：YT2715

MAPK pathway mutations in head and neck cancer affect immune microenvironments and ErbB3 signaling. Life Science Alliance 2020 Jun;3(6):e201900545 WB Human HSC-4 cell,HSC-6 cell
货号：YT2715

Nanocarrier of Pin1 inhibitor based on supercritical fluid technology inhibits cancer metastasis by blocking multiple signaling pathways Regenerative Biomaterials Dayun Yang WB Human HuH7 cell,HepG2 cell
货号：YT2715

Ambient NO2 hinders neutrophil extracellular trap formation in rats: Assessment of the role of neutrophil autophagy. Fang Xiao WB Rat 1:1000 neutrophils
货号：YT2715

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs