• 靶点：
• HXK I

• 简介：
• >>Glycolysis / Gluconeogenesis;>>Fructose and mannose metabolism;>>Galactose metabolism;>>Starch and sucrose metabolism;>>Amino sugar and nucleotide sugar metabolism;>>Neomycin, kanamycin and gentamicin biosynthesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of nucleotide sugars;>>HIF-1 signaling pathway;>>Insulin signaling pathway;>>Type II diabetes mellitus;>>Carbohydrate digestion and absorption;>>Shigellosis;>>Central carbon metabolism in cancer

• 基因名称：
• HK1

• 蛋白名称：
• Hexokinase-1

• Human Gene Id：
• 3098

• Human Swiss Prot No：
• P19367

• Mouse Swiss Prot No：
• P17710

• Rat Gene Id：
• 25058

• Rat Swiss Prot No：
• P05708

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human HXK1. AA range:31-80

• 特异性：
• HXK I Polyclonal Antibody detects endogenous levels of HXK I protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HK1;Hexokinase-1;Brain form hexokinase;Hexokinase type I;HK I

• 实测条带：
• 109kD

• 背景：
• Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016],

• 功能：
• catalytic activity:ATP + D-hexose = ADP + D-hexose 6-phosphate.,disease:Defects in HK1 are the cause of hexokinase deficiency [MIM:235700]. Hexokinase deficiency is a rare autosomal recessive disease with nonspherocytic hemolytic anemia as the predominant clinical feature.,domain:The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.,enzyme regulation:Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.,miscellaneous:In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).,online information:Hexokinase entry,pathway:Carbohydrate metabolism; hexose metabolism.,similarity:Belongs to the hexokinase family.,subcellular location:Its hydrophobic N-ter

• 细胞定位：
• Mitochondrion outer membrane ; Peripheral membrane protein . Cytoplasm, cytosol . The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331). .

• 组织表达：
• Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502).

PKM2 regulates neural invasion of and predicts poor prognosis for human hilar cholangiocarcinoma. Molecular Cancer 2015 Nov 14 IHC Human 1:100 hilar cholangiocarcinoma (HC) tissue
货号：YT2265

Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo. LABORATORY INVESTIGATION Lab Invest. 2015 Nov;96(1):37-48 IHC Human 1:100 hilar cholangiocarcinoma (HC) tissue
货号：YT2265

The role of p38MAPK signal pathway in the neuroprotective mechanism of limb postconditioning against rat cerebral ischemia/reperfusion injury. JOURNAL OF THE NEUROLOGICAL SCIENCES J Neurol Sci. 2015 Oct;357:270 IHC Human 1:100 gastric cancer tissue,gastric normal epithelium
货号：YT2265

Expression patterns of three regulation enzymes in glycolysis in esophageal squamous cell carcinoma: association with survival. MEDICAL ONCOLOGY Med Oncol. 2014 Sep;31(9):1-8 IHC Human 1:100 esophageal squamous cell carcinoma (ESCC) tissue
货号：YT2265

Expression patterns of three regulation enzymes in glycolysis in esophageal squamous cell carcinoma: association with survival. MEDICAL ONCOLOGY Med Oncol. 2014 Sep;31(9):1-8 IHC Human 1:100 esophageal squamous cell carcinoma (ESCC) tissue
货号：YT2265

Overexpression of metabolic markers HK1 and PKM2 contributes to lymphatic metastasis and adverse prognosis in Chinese gastric cancer. International Journal of Clinical and Experimental Pathology Int J Clin Exp Patho. 2015; 8(8): 9264–9271 IHC Human 1:100 gastric cancer tissue
货号：YT2265

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