HSP90A Polyclonal Antibody

    • 货号:YT2257
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • HSP 90
      • 简介:
      • >>Protein processing in endoplasmic reticulum;>>PI3K-Akt signaling pathway;>>Necroptosis;>>Antigen processing and presentation;>>NOD-like receptor signaling pathway;>>IL-17 signaling pathway;>>Th17 cell differentiation;>>Progesterone-mediated oocyte maturation;>>Estrogen signaling pathway;>>Salmonella infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>Prostate cancer;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis
      • 基因名称:
      • HSP90AA1
      • 蛋白名称:
      • Heat shock protein HSP 90-alpha
      • Human Gene Id:
      • 3320
      • Human Swiss Prot No:
      • P07900
      • Mouse Swiss Prot No:
      • P07901
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human HSP90A. AA range:683-732
      • 特异性:
      • HSP90A Polyclonal Antibody detects endogenous levels of HSP90A protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • HSP90AA1;HSP90A;HSPC1;HSPCA;Heat shock protein HSP 90-alpha;Heat shock 86 kDa;HSP 86;HSP86;Renal carcinoma antigen NY-REN-38
      • 实测条带:
      • 85kD
      • 背景:
      • The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],
      • 功能:
      • caution:Despite classification as a pseudogene, the existence of this protein is supported by unambiguous mass spectrometry evidence.,function:Molecular chaperone.,function:Molecular chaperone. Has ATPase activity.,similarity:Belongs to the heat shock protein 90 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Interacts with AHSA1, SMYD3 and TOM34. Interacts with FNIP1 and HSF1.,
      • 细胞定位:
      • Nucleus . Cytoplasm . Melanosome . Cell membrane . Mitochondrion . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      • 组织表达:
      • Adult brain,Brain,Brain cortex,Breast,Cajal-Retzius

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      • 产品图片
      • Immunofluorescence analysis of Hela cell. 1,HSP90A Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Active Caspase-3 Monoclonal Antibody(5E1)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western Blot analysis of various cells using HSP90A Polyclonal Antibody diluted at 1:2000
      • Immunofluorescence analysis of NIH/3T3 cells, using HSP90A Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HSP90A Antibody. The picture on the right is blocked with the synthesized peptide.