p47-phox (phospho Ser328) Polyclonal Antibody

    • 货号:YP1018
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat;Cow
      • 靶点:
      • p47-phox
      • 简介:
      • >>Chemokine signaling pathway;>>Phagosome;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Prion disease;>>Leishmaniasis;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis
      • 基因名称:
      • NCF1
      • 蛋白名称:
      • Neutrophil cytosol factor 1
      • Human Swiss Prot No:
      • P14598
      • Mouse Swiss Prot No:
      • Q09014
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328. AA range:301-350
      • 特异性:
      • Phospho-p47-phox (S328) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S328.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:10000. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • NCF1;NOXO2;SH3PXD1A;Neutrophil cytosol factor 1;NCF-1;47 kDa autosomal chronic granulomatous disease protein;47 kDa neutrophil oxidase factor;NCF-47K;Neutrophil NADPH oxidase factor 1;Nox organizer 2;Nox-organizing protein 2;SH3
      • 分子量:
      • 45kD
      • 背景:
      • The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.,function:NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).,online information:NCF1 deficiency database,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with NOXA1.,
      • 细胞定位:
      • Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side .
      • 组织表达:
      • Detected in peripheral blood monocytes and neutrophils (at protein level).

      Free fatty acids promote degranulation of azurophil granules in neutrophils by inducing production of NADPH oxidase-derived reactive oxygen species in cows with subclinical ketosis J Dairy Sci. 2022 Jan;:. WB Cow 1:1000
      货号:YP1018

      β-Hydroxybutyrate impairs the release of bovine neutrophil extracellular traps through inhibiting phosphoinositide 3-kinase–mediated nicotinamide adenine dinucleotide phosphate oxidase reactive oxygen species production J Dairy Sci. 2022 Feb;:. WB Bovine 1:1000
      货号:YP1018

      • 产品图片
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody. The picture on the right is blocked with the phospho peptide.