• 靶点：
• 4E-BP1

• 简介：
• >>EGFR tyrosine kinase inhibitor resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Cellular senescence;>>Insulin signaling pathway;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Herpes simplex virus 1 infection;>>Chemical carcinogenesis - receptor activation;>>Acute myeloid leukemia;>>Choline metabolism in cancer

• 基因名称：
• EIF4EBP1

• 蛋白名称：
• Eukaryotic translation initiation factor 4E-binding protein 1

• Human Gene Id：
• 1978

• Human Swiss Prot No：
• Q13541

• Mouse Gene Id：
• 13685

• Mouse Swiss Prot No：
• Q60876

• Rat Gene Id：
• 116636

• Rat Swiss Prot No：
• Q62622

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Ser64. AA range:30-79

• 特异性：
• Phospho-4E-BP1 (S64) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at S64.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• EIF4EBP1;Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1;eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1;PHAS-I

• 实测条带：
• 15kD

• 背景：
• eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008],

• 功能：
• function:Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.,PTM:Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eIF4E-binding protein family.,subunit:Nonphosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.,

• 细胞定位：
• nucleoplasm,cytoplasm,cytosol,protein complex,

• 组织表达：
• Colon,Epithelium,Lung,Placenta,Platelet,

Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening. Frontiers in Pharmacology Front Pharmacol. 2021; 12: 658197 WB Human HuH-7 cells,SMMC-7721 cells
货号：YP0618

Baicalin inhibits autophagy induced by influenza A virus H3N2. ANTIVIRAL RESEARCH Antivir Res. 2015 Jan;113:62 WB Human A549 cell , Ana-1 cell
货号：YP0618

RAPTOR promotes colorectal cancer proliferation by inducing mTORC1 and upregulating ribosome assembly factor URB1. Cancer Medicine Cancer Med-Us. 2020 Feb;9(4):1529-1543 WB Human 1:1500，1:200 colorectal cancer (CRC) tissue RKO cell, SW480 cell, HCT116 cell,LoVo cell
货号：YP0618

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