Cdk2/Cdc2 (phospho Thr160) Polyclonal Antibody

    • 货号:YP0592
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Cdk2/Cdc2
      • 简介:
      • >>FoxO signaling pathway;>>Cell cycle;>>Oocyte meiosis;>>p53 signaling pathway;>>PI3K-Akt signaling pathway;>>Cellular senescence;>>Progesterone-mediated oocyte maturation;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Prostate cancer;>>Small cell lung cancer;>>Gastric cancer
      • 基因名称:
      • CDK2
      • 蛋白名称:
      • Cyclin-dependent kinase 2
      • Human Gene Id:
      • 1017
      • Human Swiss Prot No:
      • P24941
      • Mouse Swiss Prot No:
      • P97377
      • 免疫原:
      • Synthesized phospho-peptide around the phosphorylation site of human Cdk2/Cdc2 (phospho Thr160)
      • 特异性:
      • Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody detects endogenous levels of Cdk2/Cdc2 protein only when phosphorylated at T160.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CDK2;CDKN2;Cyclin-dependent kinase 2;Cell division protein kinase 2;p33 protein kinase
      • 实测条带:
      • 34kD
      • 背景:
      • cyclin dependent kinase 2(CDK2) Homo sapiens This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],
      • 功能:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-160 activates it.,function:Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1.,
      • 细胞定位:
      • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).
      • 组织表达:
      • Epithelium,Lung,Placenta,

      Functional characterization of a potent anti-tumor polysaccharide in a mouse model of gastric cancer. LIFE SCIENCES 2019 Jan 03 WB Mouse 1:500 MFC cell
      货号:YP0592

      • 产品图片
      • Western Blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1:500
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDK2/CDC2 (Phospho-Thr160) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast cancer, using CDK2/CDC2 (Phospho-Thr160) Antibody. The picture on the right is blocked with the CDK2/CDC2 (Phospho-Thr160) peptide.