FoxO1 (phospho Ser256) Polyclonal Antibody

    • 货号:YP0113
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat;Drosophila
      • 靶点:
      • FoxO1
      • 简介:
      • >>FoxO signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Cellular senescence;>>Insulin signaling pathway;>>Thyroid hormone signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Alcoholic liver disease;>>Shigellosis;>>Human papillomavirus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Prostate cancer
      • 基因名称:
      • FOXO1
      • 蛋白名称:
      • Forkhead box protein O1
      • Human Gene Id:
      • 2308
      • Human Swiss Prot No:
      • Q12778
      • Mouse Swiss Prot No:
      • Q9R1E0
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272
      • 特异性:
      • Phospho-FoxO1 (S256) Polyclonal Antibody detects endogenous levels of FoxO1 protein only when phosphorylated at S256.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • FOXO1;FKHR;FOXO1A;Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma
      • 实测条带:
      • 82kD
      • 背景:
      • This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.,function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles betw
      • 细胞定位:
      • Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation
      • 组织表达:
      • Ubiquitous.

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      • 产品图片
      • Immunofluorescence analysis of Hela cell. 1,FoxO1 (phospho Ser256) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Active Caspase-3 Monoclonal Antibody(5E1)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western Blot analysis of HELA cells using Phospho-FoxO1 (S256) Polyclonal Antibody diluted at 1:1000
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FKHR (Phospho-Ser256) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHR (Phospho-Ser256) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HeLa cells treated with EGF+Serum, using FKHR (Phospho-Ser256) Antibody. The lane on the right is blocked with the phospho peptide.