T7-Tag Monoclonal Antibody(1H5)
- 货号：YM3012
- 应用：WB
- 种属：Species independent
- 免疫原：
- Synthetic Peptide of T7-Tag
- 特异性：
- The antibody detects T7 tag fusion proteins.
- 组成：
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 纯化工艺：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景：
- T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10, which encodes a T7 major capsid protein. The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems.
- 0.5ug T7 fusion protein+ Primary antibody dilution at
1) 1:3000
2) 1:5000
3) 1:10000
