CHD3(C-term) mouse mAb

    • 货号:YM1249
    • 应用:WB;ICC;IP
    • 种属:Human;Mouse;Rat
      • 靶点:
      • CHD3
      • 基因名称:
      • chd3
      • Human Gene Id:
      • 1107
      • Human Swiss Prot No:
      • Q12873
      • 免疫原:
      • Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
      • 特异性:
      • This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • wb 1:1000 icc 1:400
      • 纯化工艺:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • 226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-alpha;Prp7;Prp9 1;ZFH;Zinc finger helicase;zinc-finger helicase (Snf2-like).
      • 实测条带:
      • 260kD
      • 背景:
      • This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.,function:Probable transcription regulator.,sequence caution:Differs from position 1967 onward for unknown reasons.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts via its C-terminal region with HABP4.,tissue specificity:Widely expressed.,
      • 细胞定位:
      • Nucleus, PML body . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase and mitosis. .
      • 组织表达:
      • Widely expressed.

      A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis MOLECULAR CELL Kaiwen Bao IF Human HeLa cell
      货号:YM1249

      • 产品图片
      • Western blot detection of CHD3 (C-terminus) in NTERA2,3T3,C6,K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.
      • Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).
      • Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.