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Ikaros Monoclonal Antibody

货号：YM1052
应用：WB
种属：Human;Mouse;Dog

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靶点：
Ikaros

基因名称：
IKZF1

蛋白名称：
DNA-binding protein Ikaros

Human Gene Id：
10320

Human Swiss Prot No：
Q13422

Mouse Swiss Prot No：
Q03267

免疫原：
Purified recombinant human Ikaros (C-terminus) protein fragments expressed in E.coli.

特异性：
Ikaros Monoclonal Antibody detects endogenous levels of Ikaros protein.

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Monoclonal, Mouse

稀释：
WB 1:1000 - 1:2000. Not yet tested in other applications.

纯化工艺：
Affinity purification

浓度：
1 mg/ml

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
IKZF1;IK1;IKAROS;LYF1;ZNFN1A1;DNA-binding protein Ikaros;Ikaros family zinc finger protein 1;Lymphoid transcription factor LyF-1

分子量：
58kD

背景：
This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding

功能：
function:Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development.,similarity:Belongs to the Ikaros C2H2-type zinc-finger protein family.,similarity:Contains 6 C2H2-type zinc fingers.,subunit:Interacts with IKZF4 AND IKZF5.,tissue specificity:Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.,

细胞定位：
Nucleus . In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events. .; [Isoform Ik2]: Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity). .; [Isoform Ik6]: Cytoplasm .

组织表达：
Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.

Transcription factor IKZF1 is degraded during the apoptosis of multiple myeloma cells induced by kinase inhibition. FEBS LETTERS 2015 Jul 13 WB Human HEK293T cell
货号：YM1052