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CD4 Monoclonal Antibody

货号：YM0124
应用：FCM;ELISA
种属：Human

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靶点：
CD4

简介：
>>Viral life cycle - HIV-1;>>Cytokine-cytokine receptor interaction;>>Cell adhesion molecules;>>Antigen processing and presentation;>>Hematopoietic cell lineage;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Yersinia infection;>>Human T-cell leukemia virus 1 infection;>>Human immunodeficiency virus 1 infection;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Primary immunodeficiency

基因名称：
CD4

蛋白名称：
T-cell surface glycoprotein CD4

Human Gene Id：
920

Human Swiss Prot No：
P01730

Mouse Swiss Prot No：
P06332

免疫原：
Purified recombinant fragment of human CD4 expressed in E. Coli.

特异性：
CD4 Monoclonal Antibody detects endogenous levels of CD4 protein.

组成：
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

来源：
Monoclonal, Mouse

稀释：
Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.

纯化工艺：
Affinity purification

浓度：
0.5 mg/ml

储存：
-15°C to -25°C/1 year(Do not lower than -25°C)

其他名称：
CD4;T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;CD antigen CD4

背景：
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010],

功能：
function:Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.,miscellaneous:Primary receptor for HIV-1.,online information:CD4 entry,PTM:Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,similarity:Contains 3 Ig-like C2-type (immunoglobulin-like) domains.,subcellular location:Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.,subunit:Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to t

细胞定位：
Cell membrane ; Single-pass type I membrane protein . Localizes to lipid rafts (PubMed:12517957, PubMed:9168119). Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

组织表达：
Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.

Effect of montelukast combined with methylprednisolone for the treatment of mycoplasma pneumonia:. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 2019 May 09 FC Human T cell
货号：YM0124