E2F-1 (Acetyl-K117) Polyclonal Antibody

    • 货号:YK0087
    • 应用:WB;ELISA
    • 种属:Human:K117;Mouse:K112;Rat:K115
      • 靶点:
      • E2F-1
      • 简介:
      • >>Endocrine resistance;>>Cell cycle;>>Mitophagy - animal;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - receptor activation;>>Pancreatic cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
      • 基因名称:
      • E2F1 RBBP3
      • 蛋白名称:
      • E2F-1
      • Human Gene Id:
      • 1869
      • Human Swiss Prot No:
      • Q01094
      • Mouse Swiss Prot No:
      • Q61501
      • 免疫原:
      • Synthesized Acetyl peptide derived from human E2F-1. at AA range: K117
      • 特异性:
      • This antibody detects endogenous levels of E2F-1 at Human:K117;Mouse:K112;Rat:K115, It doesn't reacte with total protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • wb dilution 1:2000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • Transcription factor E2F1 (E2F-1) (PBR3) (Retinoblastoma-associated protein 1) (RBAP-1) (Retinoblastoma-binding protein 3) (RBBP-3) (pRB-binding protein E2F-1)
      • 实测条带:
      • 60kD
      • 背景:
      • The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
      • 功能:
      • function:Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.,PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, ren
      • 细胞定位:
      • Nucleus .
      • 组织表达:
      • Brain,Epithelium,Pancreas,Skin,

      The circular RNA circZFR phosphorylates Rb promoting cervical cancer progression by regulating the SSBP1/CDK2/cyclin E1 complex. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH J Exp Clin Canc Res. 2021 Dec;40(1):1-18 WB Mouse,Human 1:2000 Cervical cancer tissues HeLa cell,SiHa cells
      货号:YK0087

      • 产品图片
      • Western Blot analysis of 1,hela 2,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)