HA-tag Polyclonal Antibody

    • 货号:YG0003
    • 应用:WB;ELISA
    • 种属:Species independent
      • 靶点:
      • HA-tag
      • 基因名称:
      • HA Tag
      • 蛋白名称:
      • HA-Tag
      • 免疫原:
      • HA synthetic peptide conjugated to KLH.
      • 特异性:
      • HA-tag Polyclonal Antibody detects HA-tagged recombinant proteins or HA-tagged proteins overexpressed in cells.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:1000 - 1:3000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • HA epitope tag;hemagglutinin
      • 实测条带:
      • 15kD
      • 背景:
      • The HA tag (Y-P-Y-D-V-P-D-Y-A) is derived from an epitope (amino acids 98-106) of the influenza hemagglutinin (HA) protein. HA tag is used as a general epitope tag in expression vectors for many recombinant proteins. The presence of HA tag facilitates the detection, isolation and purification of recombinant fusion proteins.

      Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2017 Mar;484:753 CoIP Human LX-2 cell,293T cell

      Acyl-CoA-Binding Protein ACBP1 Modulates Sterol Synthesis during Embryogenesis. PLANT PHYSIOLOGY Plant Physiol. 2017 Jun;174(3):1420-1435 WB Arabidopsis Thaliana 1:5000

      PRMT5 promotes epithelial‐mesenchymal transition via EGFR‐β‐catenin axis in pancreatic cancer cells. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2019 Dec 18 WB Human PaTu8988 cell, SW1990 cell

      Chen, Zhida, et al. "Silencing Herg1 by shRNA inhibits the proliferation and invasion of osteosarcoma in vivo and in vitro." (2021).

      The BnTFL1–BnGF14nu–BnFD module regulates flower development and plant architecture in Brassica napus. Crop Journal Mingxun Chen CoIP Escherichia coli 1:2000

      • 产品图片
      • Western Blot analysis using HA-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing HA tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • IP analysis of HA-tag Polyclonal Antibody