- 靶点:
- HA-tag
- 基因名称:
- HA Tag
- 蛋白名称:
- HA-Tag
- 免疫原:
- HA synthetic peptide conjugated to KLH.
- 特异性:
- HA-tag Polyclonal Antibody detects HA-tagged recombinant proteins or HA-tagged proteins overexpressed in cells.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:1000 - 1:3000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- HA epitope tag;hemagglutinin
- 实测条带:
- 15kD
- 背景:
- The HA tag (Y-P-Y-D-V-P-D-Y-A) is derived from an epitope (amino acids 98-106) of the influenza hemagglutinin (HA) protein. HA tag is used as a general epitope tag in expression vectors for many recombinant proteins. The presence of HA tag facilitates the detection, isolation and purification of recombinant fusion proteins.
Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Biochem Bioph Res Co. 2017 Mar;484:753 CoIP Human LX-2 cell,293T cell
货号:YG0003
Acyl-CoA-Binding Protein ACBP1 Modulates Sterol Synthesis during Embryogenesis. PLANT PHYSIOLOGY Plant Physiol. 2017 Jun;174(3):1420-1435 WB Arabidopsis Thaliana 1:5000
货号:YG0003
PRMT5 promotes epithelial‐mesenchymal transition via EGFR‐β‐catenin axis in pancreatic cancer cells. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 2019 Dec 18 WB Human PaTu8988 cell, SW1990 cell
货号:YG0003
Chen, Zhida, et al. "Silencing Herg1 by shRNA inhibits the proliferation and invasion of osteosarcoma in vivo and in vitro." (2021).
货号:YG0003
The BnTFL1–BnGF14nu–BnFD module regulates flower development and plant architecture in Brassica napus. Crop Journal Mingxun Chen CoIP Escherichia coli 1:2000
货号:YG0003
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- Antibody-FAQs
- 产品图片
- Western Blot analysis using HA-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing HA tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- IP analysis of HA-tag Polyclonal Antibody