Tom20 Polyclonal Antibody
- 货号：YT4696
- 应用：WB IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Mitochondrial import receptor subunit TOM20 homolog
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human TOMM20. AA range:15-64
- 特异性：
- Tom20 Polyclonal Antibody detects endogenous levels of Tom20 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- TOMM20;KIAA0016;Mitochondrial import receptor subunit TOM20 homolog;Mitochondrial 20 kDa outer membrane protein;Outer mitochondrial membrane receptor Tom20
- 背景：
- function:Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with tom22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the tom40 translocation pore.,similarity:Belongs to the Tom20 family.,subunit:Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22.,
- 功能：
- function:Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with tom22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the tom40 translocation pore.,similarity:Belongs to the Tom20 family.,subunit:Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22.,
- 细胞定位：
- Mitochondrion outer membrane ; Single-pass membrane protein .
- 组织表达：
- Bone marrow,Brain,Cervix,Epithelium,Eye,Fibroblast,Pancreas
- Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
- Western Blot analysis of various cells using Tom20 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of the lysates from HeLa cells using TOMM20 antibody.
