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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PJA2 Polyclonal Antibody
- 货号:YT3742
- 应用:WB;ELISA;IHC
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- E3 ubiquitin-protein ligase Praja-2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human PJA2. AA range:141-190
- 特异性:
- PJA2 Polyclonal Antibody detects endogenous levels of PJA2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PJA2;KIAA0438;RNF131;E3 ubiquitin-protein ligase Praja-2;Praja2;RING finger protein 131
- 背景:
- function:Has E2-dependent E3 ubiquitin-protein ligase activity.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses.,subunit:Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2.,
- 功能:
- function:Has E2-dependent E3 ubiquitin-protein ligase activity.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses.,subunit:Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2.,
- 细胞定位:
- Cytoplasm . Cell membrane . Endoplasmic reticulum membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein . Cell junction, synapse . Cell junction, synapse, postsynaptic density . Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses (By similarity). Colocalizes with PRKAR2A and PRKAR2B in the cytoplasm and the cell membrane. .
- 组织表达:
- Brain,Embryonic kidney,Epithelium,Liver,
- Western Blot analysis of various cells using PJA2 Polyclonal Antibody
- Western Blot analysis of Jurkat cells using PJA2 Polyclonal Antibody
- Western blot analysis of lysates from Jurkat cells, using PJA2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).