HSP90β Polyclonal Antibody

    • 货号:YT2244
    • 应用:WB;IHC;IP;IF;ELISA
    • 种属:Human;Mouse;Rat;Monkey
      • 靶点:
      • HSP90B
      • 简介:
      • >>Protein processing in endoplasmic reticulum;>>PI3K-Akt signaling pathway;>>Necroptosis;>>Antigen processing and presentation;>>NOD-like receptor signaling pathway;>>IL-17 signaling pathway;>>Th17 cell differentiation;>>Progesterone-mediated oocyte maturation;>>Estrogen signaling pathway;>>Salmonella infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>Prostate cancer;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis
      • 基因名称:
      • HSP90AB1
      • 蛋白名称:
      • Heat shock protein HSP 90-beta
      • Human Gene Id:
      • 3326
      • Human Swiss Prot No:
      • P08238
      • Mouse Swiss Prot No:
      • P11499
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human HSP90B. AA range:226-275
      • 特异性:
      • HSP90β Polyclonal Antibody detects endogenous levels of HSP90β protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • HSP90AB1;HSP90B;HSPC2;HSPCB;Heat shock protein HSP 90-beta;HSP 90;Heat shock 84 kDa;HSP 84;HSP84
      • 实测条带:
      • 90kD
      • 背景:
      • This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012],
      • 功能:
      • function:Molecular chaperone. Has ATPase activity.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 90 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Interacts with TP53/p53 (By similarity). Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer.,
      • 细胞定位:
      • Cytoplasm . Melanosome . Nucleus . Secreted . Cell membrane . Dynein axonemal particle . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Translocates with BIRC2 from the nucleus to the cytoplasm during differentiation (PubMed:18239673). Secreted when associated with TGFB1 processed form (LAP) (PubMed:20599762). .
      • 组织表达:
      • Amygdala,Brain cortex,Colon,Colon carcinoma,Embryon
      • 产品图片
      • Western Blot analysis of various cells using HSP90β Polyclonal Antibody diluted at 1:2000
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunofluorescence analysis of HeLa cells, using HSP90B Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSP90B Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HUVEC/NIH/3T3/HepG2, using HSP90B Antibody. The lane on the right is blocked with the synthesized peptide.