• 靶点：
• Histamine H4 Receptor

• 简介：
• >>Neuroactive ligand-receptor interaction

• 基因名称：
• HRH4

• 蛋白名称：
• Histamine H4 receptor

• Human Gene Id：
• 59340

• Human Swiss Prot No：
• Q9H3N8

• Mouse Swiss Prot No：
• Q91ZY2

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human HRH4. AA range:221-270

• 特异性：
• Histamine H4 Receptor Polyclonal Antibody detects endogenous levels of Histamine H4 Receptor protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HRH4;GPCR105;Histamine H4 receptor;H4R;HH4R;AXOR35;G-protein coupled receptor 105;GPRv53;Pfi-013;SP9144

• 实测条带：
• 45kD

• 背景：
• Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by a family of histamine receptors, which are a subset of the G-protein coupled receptor superfamily. This gene encodes a histamine receptor that is predominantly expressed in haematopoietic cells. The protein is thought to play a role in inflammation and allergy reponses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

• 功能：
• function:The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist).,induction:Expression is either up-regulated or down-regulated upon activation of the lymphoid tissues and this regulation may depend on the presence of IL-10 or IL-13.,miscellaneous:Does not bind diphenhydramine, loratadine, ranitidine, cimetidine and chlorpheniramine. Shows modest affinity for dimaprit, impromidine, clobenpropit, thioperamide, burimamide clozapine, immepip and imetit. The order of inhibitory activity was imetit > clobenpropit > burimamide > thioperamide. Clobenpropit behaves as a partial agonist, dimaprit and impromidine show some agonist activity while clozapine behaves as a full agonist. Thioperamide shows inverse agonism (enhances cAMP activity). The order of

• 细胞定位：
• Cell membrane; Multi-pass membrane protein.

• 组织表达：
• Expressed primarily in the bone marrow and eosinophils. Shows preferential distribution in cells of immunological relevance such as T-cells, dendritic cells, monocytes, mast cells, neutrophils. Also expressed in a wide variety of peripheral tissues, including the heart, kidney, liver, lung, pancreas, skeletal muscle, prostate, small intestine, spleen, testis, colon, fetal liver and lymph node.

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