GPR120 Polyclonal Antibody
- 货号:YT1964
- 应用:WB;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Omega-3 fatty acid receptor 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human GPR120. AA range:221-270
- 特异性:
- GPR120 Polyclonal Antibody detects endogenous levels of GPR120 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- O3FAR1;GPR120;GPR129;PGR4;Omega-3 fatty acid receptor 1;G-protein coupled receptor 120;G-protein coupled receptor 129;G-protein coupled receptor GT01;G-protein coupled receptor PGR4
- 背景:
- This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012],
- 功能:
- function:Receptor for unsaturated long-chain free fatty acid (FAA). Binding of the ligand promotes the secretion of glucagon-like peptide-1 from the gastro-intestinal tract.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Abundant expression in the intestinal tract.,
- 细胞定位:
- [Isoform 1]: Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Sorted to late endosome/lysosome compartments upon internalization. .; [Isoform 2]: Cell membrane ; Multi-pass membrane protein . Endosome membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Cell projection, cilium membrane ; Multi-pass membrane protein . Sorted to late endosome/lysosome compartments upon internalization (PubMed:22282525). Specifically localizes to the primary cilium of undifferentiated adipocytes. Ciliary trafficking is TULP3-dependent. As the cilium is lost during adipogenesis, moves to the plasma membrane (Probable). .
- 组织表达:
- [Isoform 2]: The predominant isoform in human tissues. Expressed in adipose tissue, pancreatic islets, lung and brain. Expressed in alpha cells of pancreatic islets (PubMed:24742677). Expressed in primary cilia of perivascular preadipocytes of white adipose tissue (at protein level) (PubMed:31761534). ; Abundant expression in the intestinal tract. Expressed in colonic intraepithelial neuroendocrine cells.
- Western Blot analysis of various cells using GPR120 Polyclonal Antibody diluted at 1:500
- Western Blot analysis of HEPG2-UV cells using GPR120 Polyclonal Antibody diluted at 1:500
- Immunofluorescence analysis of LOVO cells, using GPR120 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from LOVO cells, using GPR120 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from 1) HEPG2-UV,2) HEK-293T, 3) LOVO cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Monoclonal Antibody(5G3) (cat:YM3030) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.