AR-β2 Polyclonal Antibody

    • 货号:YT0361
    • 应用:WB;IHC;IF;FCM;ELISA
    • 种属:Human;Rat;Mouse;
      • 靶点:
      • Adrenergic Receptor β2
      • 简介:
      • >>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction;>>Adrenergic signaling in cardiomyocytes;>>Regulation of lipolysis in adipocytes;>>Renin secretion;>>Salivary secretion;>>Chemical carcinogenesis - receptor activation
      • 基因名称:
      • ADRB2
      • 蛋白名称:
      • Beta-2 adrenergic receptor
      • Human Gene Id:
      • 154
      • Human Swiss Prot No:
      • P07550
      • Mouse Swiss Prot No:
      • P18762
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human Adrenergic Receptor beta2. AA range:321-370
      • 特异性:
      • AR-β2 Polyclonal Antibody detects endogenous levels of AR-β2 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:5000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ADRB2;ADRB2R;B2AR;Beta-2 adrenergic receptor;Beta-2 adrenoreceptor;Beta-2 adrenoceptor
      • 实测条带:
      • 47kD
      • 背景:
      • This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:Polymorphic forms of ADRB2 could impart some form of nocturnal asthma.,function:Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.,PTM:Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.,PTM:Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the recep
      • 细胞定位:
      • Cell membrane ; Multi-pass membrane protein . Early endosome . Golgi apparatus . Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325). Activated receptors are also detected within the Golgi apparatus (PubMed:27481942). .
      • 组织表达:
      • Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid,
      • 产品图片
      • Western Blot analysis of various cells using AR-β2 Polyclonal Antibody
      • Immunofluorescence analysis of HeLa cells, using Adrenergic Receptor beta2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Adrenergic Receptor beta2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from NIH/3T3 cells, using Adrenergic Receptor beta2 Antibody. The lane on the right is blocked with the synthesized peptide.