ALX3 Polyclonal Antibody

    • 货号:YT0207
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • ALX3
      • 基因名称:
      • ALX3
      • 蛋白名称:
      • Homeobox protein aristaless-like 3
      • Human Gene Id:
      • 257
      • Human Swiss Prot No:
      • O95076
      • Mouse Swiss Prot No:
      • O70137
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human ALX3. AA range:171-220
      • 特异性:
      • ALX3 Polyclonal Antibody detects endogenous levels of ALX3 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ALX3;Homeobox protein aristaless-like 3;Proline-rich transcription factor ALX3
      • 实测条带:
      • 36kD
      • 背景:
      • This gene encodes a nuclear protein with a homeobox DNA-binding domain that functions as a transcriptional regulator involved in cell-type differentiation and development. Preferential methylation of this gene's promoter is associated with advanced-stage neuroblastoma tumors. [provided by RefSeq, Jul 2008],
      • 功能:
      • function:Transcriptional regulator with a possible role in patterning of mesoderm during development.,similarity:Belongs to the paired homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,
      • 细胞定位:
      • Nucleus .
      • 组织表达:
      • Brain,Neuroblastoma,
      • 产品图片
      • Western Blot analysis of various cells using ALX3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunofluorescence analysis of HeLa cells, using ALX3 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 293 cells, using ALX3 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).