14-3-3 ζ/δ (phospho Thr232) Polyclonal Antibody

    • 货号:YP0709
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • 14-3-3 ζ/δ
      • 简介:
      • >>Cell cycle;>>Oocyte meiosis;>>PI3K-Akt signaling pathway;>>Hippo signaling pathway;>>Hepatitis C;>>Hepatitis B;>>Viral carcinogenesis
      • 基因名称:
      • YWHAZ
      • 蛋白名称:
      • 14-3-3 protein zeta/delta
      • Human Gene Id:
      • 7534
      • Human Swiss Prot No:
      • P63104
      • Mouse Swiss Prot No:
      • P63101
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta/delta around the phosphorylation site of Thr232. AA range:196-245
      • 特异性:
      • Phospho-14-3-3 ζ/δ (T232) Polyclonal Antibody detects endogenous levels of 14-3-3 ζ/δ protein only when phosphorylated at T232.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • YWHAZ;14-3-3 protein zeta/delta;Protein kinase C inhibitor protein 1;KCIP-1
      • 实测条带:
      • 28kD
      • 背景:
      • This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
      • 功能:
      • caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity.,function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,PTM:The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1.,similarity:Belongs to the 14-3-3 family.,subcellular location:Located to
      • 细胞定位:
      • Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.
      • 组织表达:
      • B-cell lymphoma,Bone marrow
      • 产品图片
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 14-3-3 zeta/delta (Phospho-Thr232) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human brain, using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from Jurkat cells treated with UV 15', using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.