XIAP (phospho Ser87) Polyclonal Antibody

    • 货号:YP0681
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • XIAP
      • 简介:
      • >>Platinum drug resistance;>>NF-kappa B signaling pathway;>>Ubiquitin mediated proteolysis;>>Apoptosis;>>Apoptosis - multiple species;>>Necroptosis;>>Focal adhesion;>>NOD-like receptor signaling pathway;>>Toxoplasmosis;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>Small cell lung cancer
      • 基因名称:
      • XIAP
      • 蛋白名称:
      • E3 ubiquitin-protein ligase XIAP
      • Human Gene Id:
      • 331
      • Human Swiss Prot No:
      • P98170
      • Mouse Swiss Prot No:
      • Q60989
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human XIAP around the phosphorylation site of Ser87. AA range:53-102
      • 特异性:
      • Phospho-XIAP (S87) Polyclonal Antibody detects endogenous levels of XIAP protein only when phosphorylated at S87.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • XIAP;API3;BIRC4;IAP3;E3 ubiquitin-protein ligase XIAP;Baculoviral IAP repeat-containing protein 4;IAP-like protein;ILP;hILP;Inhibitor of apoptosis protein 3;IAP-3;hIAP-3;hIAP3;X-linked inhibitor of apoptosis protein;X-linked I
      • 实测条带:
      • 57kD
      • 背景:
      • This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011],
      • 功能:
      • disease:Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.,domain:The first BIR domain is involved in interaction with MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.,function:Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, lead
      • 细胞定位:
      • Cytoplasm. Nucleus. TLE3 promotes its nuclear localization.
      • 组织表达:
      • Expressed in colonic crypts (at protein level) (PubMed:30389919). Ubiquitous, except peripheral blood leukocytes (PubMed:8654366).

      Brucella Outer Membrane Lipoproteins 19 and 16 Differentially Induce Interleukin-18 Response or Pyroptosis in Human Monocytic Cells. JOURNAL OF INFECTIOUS DISEASES J Infect Dis. 2021 Dec;224(12):2148-2159 WB Human THP-1 cells
      货号:YP0681

      • 产品图片
      • Western Blot analysis of HepG2 cells using Phospho-XIAP (S87) Polyclonal Antibody diluted at 1:500
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using XIAP (Phospho-Ser87) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using XIAP (Phospho-Ser87) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HepG2 cells treated with Anisomycin 25ug/ml 30', using XIAP (Phospho-Ser87) Antibody. The lane on the right is blocked with the phospho peptide.