IKKβ (phospho Tyr199) Polyclonal Antibody

    • 货号:YP0654
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • IKBKB
      • 简介:
      • >>Antifolate resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Chemokine signaling pathway;>>NF-kappa B signaling pathway;>>FoxO signaling pathway;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>Cytosolic DNA-sensing pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>TNF signaling pathway;>>Neurotrophin signaling pathway;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease;>>Alzheimer disease;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yer
      • 基因名称:
      • IKBKB
      • 蛋白名称:
      • Inhibitor of nuclear factor kappa-B kinase subunit beta
      • Human Gene Id:
      • 3551
      • Human Swiss Prot No:
      • O14920
      • Mouse Swiss Prot No:
      • O88351
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr199. AA range:166-215
      • 特异性:
      • Phospho-IKKβ (Y199) Polyclonal Antibody detects endogenous levels of IKKβ protein only when phosphorylated at Y199.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • IKBKB;IKKB;Inhibitor of nuclear factor kappa-B kinase subunit beta;I-kappa-B-kinase beta;IKK-B;IKK-beta;IkBKB;I-kappa-B kinase 2;IKK2;Nuclear factor NF-kappa-B inhibitor kinase beta;NFKBIKB
      • 实测条带:
      • 85kD
      • 背景:
      • The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011],
      • 功能:
      • catalytic activity:ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].,function:Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.,PTM:Ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues.,PTM:Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.,PTM:Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.,similarity:Belongs to the p
      • 细胞定位:
      • Cytoplasm . Nucleus . Membrane raft . Colocalized with DPP4 in membrane rafts. .
      • 组织表达:
      • Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
      • 产品图片
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IKK-beta (Phospho-Tyr199) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IKK-beta (Phospho-Tyr199) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5', using IKK-beta (Phospho-Tyr199) Antibody. The lane on the right is blocked with the phospho peptide.