FADD (phospho Ser194) Polyclonal Antibody
- 货号:YP0628
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 靶点:
- FADD
- 简介:
- >>Platinum drug resistance;>>Apoptosis;>>Apoptosis - multiple species;>>Necroptosis;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>IL-17 signaling pathway;>>TNF signaling pathway;>>Alcoholic liver disease;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Chagas disease;>>Tuberculosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Pathways in cancer
- 基因名称:
- FADD
- 蛋白名称:
- Protein FADD
- Human Gene Id:
- 8772
- Human Swiss Prot No:
- Q13158
- Mouse Swiss Prot No:
- Q61160
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human FADD around the phosphorylation site of Ser194. AA range:159-208
- 特异性:
- Phospho-FADD (S194) Polyclonal Antibody detects endogenous levels of FADD protein only when phosphorylated at S194.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- FADD;MORT1;GIG3;Protein FADD;FAS-associated death domain protein;FAS-associating death domain-containing protein;Growth-inhibiting gene 3 protein;Mediator of receptor induced toxicity
- 实测条带:
- 28kD
- 背景:
- The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008],
- 功能:
- domain:Contains a death domain involved in the binding of the corresponding domain within Fas receptor.,function:Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.,PTM:Phosphorylated.,similarity:Contains 1 death domain.,similarity:Contains 1 DED (death effector) domain.,subunit:Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and LRDD.,tissue specificity:Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.,
- 细胞定位:
- cytoplasm,cytosol,plasma membrane,death-inducing signaling complex,CD95 death-inducing signaling complex,neuron projection,cell body,membrane raft,ripoptosome,
- 组织表达:
- Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
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- Antibody-FAQs
- 产品图片
- Western blot analysis of MOUSE-KIDNEY MOUSE-BRAIN MOUSE-SPLEEN MOUSE-LUNG using p-FADD (S194) antibody. Antibody was diluted at 1:1000
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FADD (Phospho-Ser194) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from HeLa cells treated with Paclitaxel 1uM 60', using FADD (Phospho-Ser194) Antibody. The lane on the right is blocked with the phospho peptide.