CD43 (ABT-CD43) mouse mAb
- 货号:YM6154
- 应用:IHC;WB;ELISA
- 种属:Human
- 简介:
- >>Cell adhesion molecules
- 蛋白名称:
- Leukosialin (Galactoglycoprotein) (GALGP) (Leukocyte sialoglycoprotein) (Sialophorin) (CD antigen CD43)
- 免疫原:
- Synthesized peptide derived from human CD43 AA range: 300-400
- 特异性:
- This antibody detects endogenous levels of human CD43. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Mouse, Monoclonal/IgG1, Kappa
- 稀释:
- IHC 1:200-400, WB 1:200-1000, ELISA 1:5000-20000
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011],
- 功能:
- disease:CD43 expression is defective on the T-cells of males with the immunodeficiency Wiskott-Aldrich syndrome. Affected males are susceptible to opportunistic infections and do not respond to polysaccharide antigens, reflecting defects in cytotoxic and helper T-cell functions.,function:One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.,PTM:Glycosylated; has a high c
- 组织表达:
- Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
- K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD43(ABT-CD43) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.
Lane 1: K562
Predicted band size: 40kDa
Observed band size: 90kDa
- Human appendix tissue was stained with Anti-CD43 (ABT-CD43) Antibody
- Human lymphoma tissue was stained with Anti-CD43 (ABT-CD43) Antibody
- Human lymphoma tissue was stained with Anti-CD43 (ABT-CD43) Antibody
- Human spleen tissue was stained with Anti-CD43 (ABT-CD43) Antibody
- Human tonsil tissue was stained with Anti-CD43 (ABT-CD43) Antibody