CD247 Monoclonal Antibody(4B10)

    • 货号:YM0114
    • 应用:WB;IHC;IF;FCM;ELISA
    • 种属:Human
      • 靶点:
      • CD247
      • 简介:
      • >>Natural killer cell mediated cytotoxicity;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Chagas disease;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>PD-L1 expression and PD-1 checkpoint pathway in cancer
      • 基因名称:
      • CD247
      • 蛋白名称:
      • T-cell surface glycoprotein CD3 zeta chain
      • Human Gene Id:
      • 919
      • Human Swiss Prot No:
      • P20963
      • Mouse Swiss Prot No:
      • P24161
      • 免疫原:
      • Purified recombinant fragment of human CD247 expressed in E. Coli.
      • 特异性:
      • CD247 Monoclonal Antibody detects endogenous levels of CD247 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺:
      • Affinity purification
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CD247;CD3Z;T3Z;TCRZ;T-cell surface glycoprotein CD3 zeta chain;T-cell receptor T3 zeta chain;CD antigen CD247
      • 分子量:
      • 19kD
      • 背景:
      • The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:Defects in CD247 are a cause of primary T-cell immunodeficiency [MIM:610163]. Affected individuals suffer of recurrent infections. Patients T-cell counts are very low and B-cell counts are normal.,domain:The ITAM domains mediate interaction with SHB.,function:Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.,online information:CD247 mutation db,PTM:Phosphorylated on Tyr residues after T-cell receptor triggering.,similarity:Belongs to the CD3Z/FCER1G family.,similarity:Contains 3 ITAM domains.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts w
      • 细胞定位:
      • Cell membrane ; Single-pass type I membrane protein.
      • 组织表达:
      • CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641).
      • 产品图片
      • Western Blot analysis using CD247 Monoclonal Antibody against CD247-hIgGFc transfected HEK293 cell lysate.
      • Immunohistochemistry analysis of paraffin-embedded human Thymus tissues with AEC staining using CD247 Monoclonal Antibody.
      • Immunofluorescence analysis of K562 cells using CD247 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
      • Flow cytometric analysis of Jurkat cells using CD247 Monoclonal Antibody (green) and negative control (purple).