• Target：
• Beclin 1

• Fields：
• >>Autophagy - other;>>Mitophagy - animal;>>Autophagy - animal;>>Apoptosis - multiple species;>>Apelin signaling pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Kaposi sarcoma-associated herpesvirus infection

• Gene Name：
• becn1

• Human Gene Id：
• 8678

• Human Swiss Prot No：
• Q14457

• Mouse Swiss Prot No：
• O88597

• Immunogen：
• Purified recombinant human Beclin 1 protein fragments expressed in E.coli.

• Specificity：
• This antibody detects endogenous levels of Beclin 1 and does not cross-react with related proteins.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• wb 1:1000 IP:1:50-200

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• APG6;ATG 6;ATG6;ATG6 autophagy related 6 homolog;Bcl-2-interacting protein beclin;Beclin 1 (coiled coil moesin like BCL2 interacting protein);Beclin 1 autophagy related;Beclin-1;Beclin1;BECN 1;BECN1;BECN1_HUMAN;Coiled coil myosin like BCL2 interacting protein;Coiled-coil myosin-like BCL2-interacting protein;GT197;Protein GT197;VPS 30;VPS30.

• Observed Band(KD)：
• 55kD

• Background：
• beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],

• Function：
• function:Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.,similarity:Belongs to the beclin family.,subcellular location:Expressed in dendrites and cell bodies of cerebellar Purkinje cells.,subunit:Interacts with GOPC and GRID2. Interacts with AMBRA1. Probably forms a complex with AMBRA1 and PIK3C3 (By similarity). Interacts with BCL2 and BCL2L1.,tissue specificity:Ubiquitous.,

• Subcellular Location：
• Cytoplasm . Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Endosome . Cytoplasmic vesicle, autophagosome . Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity). .; [Beclin-1-C 35 kDa]: Mitochondrion . Nucleus . Cytoplasm .; [Beclin-1-C 37 kDa]: Mitochondrion .

• Expression：
• Ubiquitous.

SIRT1 attenuates sepsis-induced acute kidney injury via Beclin1 deacetylation-mediated autophagy activation. Cell Death & Disease Cell Death Dis. 2021 Feb;12(2):1-13 IP，WB Mouse，Human Kidney HK-2 cell

Shi, Yanan, et al. "Compound Danshen dripping pills prevented leptin deficiency-induced hepatic ER stress, stimulated autophagy, and improved insulin resistance of ob/ob mice." Evidence-Based Complementary and Alternative Medicine 2020 (2020).

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