• Target：
• Actin β

• Fields：
• >>Rap1 signaling pathway;>>Phagosome;>>Apoptosis;>>Hippo signaling pathway;>>Focal adhesion;>>Adherens junction;>>Tight junction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Oxytocin signaling pathway;>>Gastric acid secretion;>>Amyotrophic lateral sclerosis;>>Bacterial invasion of epithelial cells;>>Vibrio cholerae infection;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Yersinia infection;>>Influenza A;>>Proteoglycans in cancer;>>Hepatocellular carcinoma;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Viral myocarditis;>>Fluid shear stress and atherosclerosis

• Gene Name：
• ACTB

• Protein Name：
• Actin cytoplasmic 1

• Human Gene Id：
• 60

• Human Swiss Prot No：
• P60709

• Mouse Gene Id：
• 11461

• Mouse Swiss Prot No：
• P60710

• Rat Gene Id：
• 81822

• Rat Swiss Prot No：
• P60711

• Immunogen：
• Synthetic Peptide of β-actin

• Specificity：
• The antibody detects endogenous β-actin protein.

• Formulation：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Source：
• Mouse, Monoclonal/IgG1, Kappa

• Dilution：
• IF 1:50-200 WB 1:5000-20000

• Purification：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• ACTB;Actin; cytoplasmic 1;Beta-actin;Actin β;β-Actin;Beta actin

• Observed Band(KD)：
• 43kD

• Background：
• This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],

• Function：
• disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.,function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the

• Subcellular Location：
• Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .

• Expression：
• B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car

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Medium-, long-and medium-chain-type structured lipids ameliorate high-fat diet-induced atherosclerosis by regulating inflammation, adipogenesis, and gut microbiota in ApoE−/− mice." Food & function 11.6 (2020): 5142-5155.

Zhang, Qianqian, et al. "PPARγ Agonist Pioglitazone Inhibits PDGF-induced Pulmonary Artery Smooth Muscle Cells Proliferation and Migration via Modulating TERT." (2021).

Yan, Juntang, et al. "Xiaoqinglong decoction reduces dendritic cell differentiation and regulates the Th1/Th2 balance in a mouse model of allergic asthma." Journal of Traditional Chinese Medical Sciences 7.2 (2020): 133-140.

Wang, Juan, et al. "Aged Zinc Oxide Nanoparticles Did Not Induce Cytotoxicity Through Apoptosis Signaling Pathway as Fresh NPs." (2020).

李思汉, et al. "健脾清化舒络中药对慢性萎缩性胃炎大鼠 Sonic Hedgehog 信号通路的影响." 北京中医药大学学报 44.2 (2021): 143-151.

司晓丽, et al. "芍药苷对 DSS 诱导大鼠慢性溃疡性结肠炎 IL-17 表达的影响." 中国免疫学杂志 37.08: 946.

Experimental and therapeutic medicine 17.3 (2019): 2349-2358.

7-Difluoromethoxy-5, 4′-dimethoxy-genistein attenuates macrophages apoptosis to promote plaque stability via TIPE2/TLR4 axis in high fat diet-fed ApoE−/− mice." International Immunopharmacology 96 (2021): 107477.

Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer. ONCOLOGY REPORTS Oncol Rep. 2019 Feb;41(2):989-998 WB Human 1:1000 A549/DPP cell, SKOV3/DDP cell,SGC7901/DDP cell

Facilitating colorectal cancer cell metastasis by targeted binding of long non-coding RNA ENSG00000231881 with miR-133b via VEGFC signaling pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2018 Dec 20 WB Human 1:1000 HCT-116 cell, Lovo cell

Aberrant methylation‐induced dysfunction of p16 is associated with osteoblast activation caused by fluoride. ENVIRONMENTAL TOXICOLOGY Environ Toxicol. 2019 Jan;34(1):37-47 WB Human osteoblasts

Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment. Neural Regeneration Research Neural Regen Res. 2019 Mar; 14(3): 452–461 WB Mouse 1:4000 Brain tissue

The permeability characteristics and interaction of the main components from Zhizi Bopi decoction in the MDCK cell model. XENOBIOTICA Xenobiotica. 2016;46(8):733-742 WB Dog Madin-Darby canine kidney (MDCK) cell

The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. JOURNAL OF BIOLOGICAL CHEMISTRY 2021 Jan;296: WB Human 293T cell,MDA-MB-231 cell

Taxifolin inhibits keratinocyte proliferation and ameliorates imiquimod-induced psoriasis-like mouse model via regulating cytoplasmic phospholipase A2 and PPAR-γ pathway. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2021 Oct;99:107900 WB Mouse 1:1000 Skin

Hepatitis B Virus X Protein (HBx) Suppresses Transcription Factor EB (TFEB) Resulting in Stabilization of Integrin Beta 1 (ITGB1) in Hepatocellular Carcinoma Cells. Cancers Cancers. 2021 Jan;13(5):1181 WB Human HepG2 cell，SK-Hep-1 cell，HEK-293FT cell

Bawei Chenxiang Wan Ameliorates Cardiac Hypertrophy by Activating AMPK/PPAR-α Signaling Pathway Improving Energy Metabolism. Frontiers in Pharmacology Front Pharmacol. 2021 Jun;0:1314 WB Rat Myocardial tissue

miR-301b-5p and its target gene nfatc2ip regulate inflammatory responses in the liver of rainbow trout (Oncorhynchus mykiss) under high temperature stress ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Gefeng Xu WB Fish

Effect of the FA2H Gene on cashmere fineness of Jiangnan cashmere goats based on transcriptome sequencing BMC GENOMICS Kechuan Tian WB Goat

AURKA is a prognostic potential therapeutic target in skin cutaneous melanoma modulating the tumor microenvironment, apoptosis, and hypoxia JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY Xuan Fen Zhang WB Human

Low LINC02147 expression promotes the malignant progression of oral submucous fibrosis BMC Oral Health Wenjie Li WB Human

Network Pharmacology, Molecular Docking, and Experimental Validation to Unveil the Molecular Targets and Mechanisms of Compound Fuling Granule to Treat Ovarian Cancer Oxidative Medicine and Cellular Longevity Fangfang Tao WB Human

Effects of Environmentally Relevant Concentrations of Antipsychotic Drugs (Sulpiride and Clozapine) on Serotonergic and Dopaminergic Neurotransmitter Systems in Octopus Brain Tissue Water Bo Zhang WB Octopus

Deoxynivalenol damages the intestinal barrier and biota of the broiler chickens BMC Veterinary Research Fan Ruiwen WB Chicken

Polysaccharides from Tetrastigma Hemsleyanum Diels et Gilg attenuate LPS-induced acute lung injury by modulating TLR4/COX-2/NF-κB signaling pathway BIOMEDICINE & PHARMACOTHERAPY Bixu Wang WB Mouse, Human

Mitochondrial-Targeting Vitamin B3 Ameliorates the Phenotypes of Parkinson's Disease In Vitro and In Vivo by Restoring Mitochondrial Function Advanced Therapeutics Hua Bai WB Human

miRNA-331-3p Affects the Proliferation, Metastasis, and Invasion of Osteosarcoma through SOCS1/JAK2/STAT3 Journal of Oncology Bing Wu WB Mouse

Activation of Autophagy Induces Monocrotaline-Induced Pulmonary Arterial Hypertension by FOXM1-Mediated FAK Phosphorylation LUNG Shaojun Li WB Rat

Distribution characteristics and regulation of amino acids and fatty acids in muscle and adipose tissues of sheep grown in natural grazing environment ANIMAL SCIENCE JOURNAL Jinling Cao WB Sheep

Functional and metabolic alterations of gut microbiota in children with new-onset type 1 diabetes

Suppressing Mesenchymal Stromal Cell Ferroptosis Via Targeting a Metabolism-Epigenetics Axis Corrects their Poor Retention and Insufficient Healing Benefits in the Injured Liver Milieu Advanced Science Fuyang Zhang WB Human,Rat Mesenchymal stromal/stem cells (MSCs),Adipose-derived MSCs (ADSCs)

Immunoproteasome subunit β5i promotes perifascicular muscle atrophy in dermatomyositis by upregulating RIG-I RMD Open Qinglin Peng WB Human human skeletal muscle myoblasts (HSMMs),myoblast

The novel hepatoprotective mechanisms of silibinin-phospholipid complex against d-GalN/LPS-induced acute liver injury INTERNATIONAL IMMUNOPHARMACOLOGY Yu Chen WB Mouse liver tissues

Preliminary Structural Characterization of Selenium Nanoparticle Composites Modified by Astragalus Polysaccharide and the Cytotoxicity Mechanism on Liver Cancer Cells MOLECULES Xiaoyu Jia WB Human HepG2 cell

Postsurgical wound management and prevention of triple-negative breast cancer recurrence with a pryoptosis-inducing, photopolymerizable hydrogel JOURNAL OF CONTROLLED RELEASE Sanjun Shi WB Mouse 4 T1 cell

A Liquid–Liquid Phase Separation-Related Index Associate with Biochemical Recurrence and Tumor Immune Environment of Prostate Cancer Patients INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Ning Xu WB Human prostate cancer (PCa)cell

Clinopodium chinense Kuntze ameliorates dextran sulfate sodium-induced ulcerative colitis in mice by reducing systematic inflammation and regulating metabolism JOURNAL OF ETHNOPHARMACOLOGY Dali Meng WB Mouse colon RAW264.7 cell

Euphohelides A–C, ent-Abietane-Type Norditerpene Lactones from Euphorbia helioscopia and Their Anti-Inflammatory Activities JOURNAL OF NATURAL PRODUCTS Kun Gao WB Mouse RAW264.7 cell

Local analgesia of electroacupuncture is mediated by the recruitment of neutrophils and released β-endorphins PAIN Wang, Xiao-Yu WB Rat inflammatory tissue

Effects and mechanism of Compound Qidan Formula on rats with HFpEF induced by hypertension and diabetes mellitus based on Ang Ⅱ/TGF-β1/Smads signaling pathway JOURNAL OF ETHNOPHARMACOLOGY Dazhuo Shi WB Rat myocardial tissue

MAOA suppresses the growth of gastric cancer by interacting with NDRG1 and regulating the Warburg effect through the PI3K/AKT/mTOR pathway. Li Jun WB Human 1:5000 BGC-823 cell,HGC-27 cell

Naringenin prevents NAFLD in the diet-induced C57BL/6J obesity model by regulating the intestinal barrier function and microbiota. Yifei Bian WB Mouse 1:2000 colon tissue

Cathepsin B mediates the lysosomal-mitochondrial apoptosis pathway in arsenic-induced microglial cell injury. Baofei Sun WB Mouse 1:5000 BV2 cell

Simulated microgravity altered the proliferation, apoptosis, and extracellular matrix formation of L929 fibroblasts and the transforming growth factor-β1/Smad3 signaling pathway. Yan Cui WB Mouse L929 cell

HMGB1-induced activation of ER stress contributes to pulmonary artery hypertension in vitro and in vivo. Manxiang Li WB Rat lung tissue

Y-box binding protein 1 promotes chromatin accessibility to aggravate liver fibrosis. Xinyan Zhu WB Mouse,Human 1:5000 liver tissue LX-2 cell,hepatic stellate cell

Mesoporous polydopamine based biominetic nanodrug ameliorates liver fibrosis via antioxidation and TGF-β/SMADS pathway. Fei Liu WB Mouse liver

The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis. Microbiome Zhe Zhang WB Mouse 1:3000 testis tissues GC-2 cell

Role of Txnrd3 in NiCl2-induced kidney cell apoptosis in mice: Potential therapeutic effect of melatonin. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ziwei Zhang WB Mouse 1:1500 kidney

Tanshinone ⅡA participates in the treatment of endometriosis by regulating adhesion, invasion, angiogenesis and inhibition of PI3K/Akt/mTOR signaling pathway. Molecular Medicine Reports Xin Gong WB Rat 1:5000 endometria

ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH Pengming Sun WB Human 1:2000 KLE cell，HEC-1A cell

Alternate-day fasting ameliorated anxiety-like behavior in high-fat diet-induced obese mice JOURNAL OF NUTRITIONAL BIOCHEMISTRY Hao Hu WB Mouse 1:1000 hippocampal tissue

Astrocyte and L-lactate in the anterior cingulate cortex modulate schema memory and neuronal mitochondrial biogenesis eLife Ying Li WB Rat 1:5000 brain tissue

Downhill running and caloric restriction attenuate insulin resistance associated skeletal muscle atrophy via the promotion of M2-like macrophages through TRIB3-AKT pathway FREE RADICAL BIOLOGY AND MEDICINE Lei Ai WB Mouse 1:5000 C2C12 cell,RAW264.7 cell

Inhibición de la Proliferación, Migración e Invasión de Fibroblastos Queloides por miR-183-5p Mediante la Regulación Negativa de EGR1 INTERNATIONAL JOURNAL OF MORPHOLOGY Lianbo Zhang WB Human 1:5000 Keloid fibroblasts (KFs)

Study of the Mechanism of Astragali Radix in Treating Type 2 Diabetes Mellitus and Its Renal Protection Based on Enzyme Activity, Network Pharmacology, and Experimental Verification MOLECULES Jiaming Sun WB Mouse renal tissue

Sunitinib induces cardiotoxicity through modulating oxidative stress and Nrf2-dependent ferroptosis in vitro and in vivo CHEMICO-BIOLOGICAL INTERACTIONS Xiaoyan Zhao WB Mouse,Rat heart tissue H9c2 cell

Dietary methionine supplementation during the estrous cycle improves follicular development and estrogen synthesis in rats Food & Function Xiangfang Zeng WB Rat 1:5000 Rat ovarian granulosa cells (rGCs)

Extracellular CIRP induces abnormal activation of fibroblast-like synoviocytes from patients with RA via the TLR4-mediated HDAC3 pathways INTERNATIONAL IMMUNOPHARMACOLOGY Wei Hu WB Human 1:5000 fibroblast-like synoviocytes (FLSs)

Huangqi Baihe Granules alleviate hypobaric hypoxia-induced acute lung injury in rats by suppressing oxidative stress and the TLR4/NF-κB/NLRP3 inflammatory pathway JOURNAL OF ETHNOPHARMACOLOGY Yun Su WB Rat 1:10000 lung tissue

Galectin from Trichinella spiralis alleviates DSS-induced colitis in mice by regulating the intestinal microbiota VETERINARY RESEARCH Jianbin Ye WB Mouse 1:1000 colon tissue

Lamin B1: a novel biomarker in adult and pediatric adrenocortical carcinoma ENDOCRINE-RELATED CANCER Jianming Lu WB Human 1:5000 SW13 cell,H295R cell

Uncovering the Multitarget Therapeutic Mechanism of Tong-Xie-Yao-Fang on Irritable Bowel Syndrome JOURNAL OF FOOD QUALITY Xudong Tang WB Rat 1:5000 colonic tissue

Roles of ferroptosis in type 1 diabetes induced spermatogenic dysfunction FREE RADICAL BIOLOGY AND MEDICINE Hui Jiang WB Mouse 1:3000 testis tissue GC-2 cell

Inhibition of PI3K/AKT signaling pathway prevents blood-induced heterotopic ossification of the injured tendon Journal of Orthopaedic Translation Hongwei Ouyang WB Mouse 1:1000 tendon stem/progenitor cells (TSPCs)

Nrf2-mediated redox balance alleviates LPS-induced vascular endothelial cell inflammation by inhibiting endothelial cell ferroptosis Scientific Reports Huiying Zhao WB Human 1:1000 Human umbilical vein endothelial cells (HUVEC)

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