GAPDH Monoclonal Antibody(2B8)

    • Catalog No.:YM3029
    • Applications:WB
    • Reactivity:Human; Mouse; Rat;Rabbit
      • Target:
      • GAPDH
      • Fields:
      • >>Glycolysis / Gluconeogenesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of amino acids;>>HIF-1 signaling pathway;>>Alzheimer disease;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Diabetic cardiomyopathy
      • Gene Name:
      • GAPDH
      • Protein Name:
      • Glyceraldehyde-3-phosphate dehydrogenase
      • Human Gene Id:
      • 2597
      • Human Swiss Prot No:
      • P04406
      • Mouse Swiss Prot No:
      • P16858
      • Immunogen:
      • Synthetic Peptide of human GAPDH AA range: 200-300
      • Specificity:
      • The antibody detects endogenous GAPDH protein.
      • Formulation:
      • PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
      • Source:
      • Monoclonal, Mouse
      • Dilution:
      • WB 1:2000-10000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • GAPDH;GAPD;CDABP0047;OK/SW-cl.12;Glyceraldehyde-3-phosphate dehydrogenase;GAPDH;Peptidyl-cysteine S-nitrosylase GAPDH
      • Observed Band(KD):
      • 37kD
      • Background:
      • glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferri
      • Function:
      • catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.,function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence caution:Differs quite extensively.,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase fami
      • Subcellular Location:
      • Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). .
      • Expression:
      • Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu

      DDI2 promotes tumor metastasis and resists antineoplastic drugs-induced apoptosis in colorectal cancer APOPTOSIS Lei, Lei, Cao, Qing, An, Guoyan, Lv, Yuejiao, Tang, Juan, Yang, Jin WB Human LoVo cell, SW480 cell, HCT-116 cell

      Zhongfeng Capsules protects against cerebral ischemia–reperfusion injury via mediating the phosphoinositide 3-kinase/Akt and toll-like receptor 4/nuclear factor kappa B signaling pathways by regulating neuronal apoptosis and inflammation WB Rat 1:2000 brain tissue/

      Shaoyao-Gancao Decoction Promoted Microglia M2 Polarization via the IL-13-Mediated JAK2/STAT6 Pathway to Alleviate Cerebral Ischemia-Reperfusion Injury WB Rat 1:5000 penumbra tissue/

      Lower Expression of TWEAK is Associated with Poor Survival and Dysregulate TIICs in Lung Adenocarcinoma WB Human lung epithelium tissue/HBE ,NSCLC cell lines A549, H1299, H358, SPCA1, PC9, HCC827, and H1993 cells

      Vitamin D3 Nutritional Status Affects Gut Health of Salmonella-Challenged Laying Hens WB Chicken jejunal mucosa/

      Sparstolonin B inhibits pancreatic adenocarcinoma through the NF-κB signaling pathway WB Human /Panc-1 cell, SW1990 cell

      Modulating Effect of Paeonol on Piglets With Ulcerative Colitis. Frontiers in Nutrition2022; 9: 846684. Pig 1:10000 colon tissue

      Irisin Suppresses Nicotine-Mediated Atherosclerosis by Attenuating Endothelial Cell Migration, Proliferation, Cell Cycle Arrest, and Cell Senescence. Frontiers in Cardiovascular Medicine2022; 9: 851603. Human 1:5000 HUVECs

      Referred Somatic Hyperalgesia Mediates Cardiac Regulation by the Activation of Sympathetic Nerves in a Rat Model of Myocardial Ischemia. Neuroscience Bulletin2022 Apr;38(4):386-402. Rat 1:20000 spinal segments,hypersensitive skin

      PDK4 Constitutes a Novel Prognostic Biomarker and Therapeutic Target in Gastric Cancer. Diagnostics2022 May;12(5):1101. Human 1:1000 HGC27 cell, NCI-N87 cell, MKN45 cell, AGS cell,GES-1 cell

      ANGPTL3 impacts proteinuria and hyperlipidemia in primary nephrotic syndrome. Lipids in Health and Disease2022 Dec;21(1):1-13. Mouse liver

      Inactivation of EGLN3 hydroxylase facilitates Erk3 degradation via autophagy and impedes lung cancer growth Oncogene. 2022 Feb;:1-15. WB Mouse,Human kidney,heart

      α-Hederin Inhibits the Proliferation of Hepatocellular Carcinoma Cells via Hippo-Yes-Associated Protein Signaling Pathway. Front Oncol. 2022 Mar;12:839603-839603. WB Human,Mouse 1:5000 HepG2-xenotransplant tumor

      Kaempferol Can Reverse the 5-Fu Resistance of Colorectal Cancer Cells by Inhibiting PKM2-Mediated Glycolysis Int J Mol Sci. 2022 Jan;23(7):3544. WB Human 1:2000

      DLEU7-AS1 promotes renal cell cancer by silencing the miR-26a-5p/coronin-3 axis Clin Kidney J. 2022 Feb;:. WB Human

      Positive effects of selenium supplementation on selenoprotein S expression and cytokine status in a murine model of acute liver injury J Trace Elem Med Bio. 2022 May;71:126927. WB Mouse liver

      Angiopoietin-like protein 4 promotes hyperlipidemia-induced renal injury by down-regulating the expression of ACTN4 Biochem Bioph Res Co. 2022 Mar;595:69. IHC Mouse Renal

      Electroacupuncture Prevents the Depression-Like Behavior by Inhibiting the NF-κB/NLRP3 Inflammatory Pathway in Hippocampus of Mice Subjected to Chronic Mild Stress Neuropsychobiology. ;:1-9. WB Mouse 1:1000 hippocampus

      Selected lactobacilli strains inhibit inflammation in LPS-induced RAW264.7 macrophages by suppressing the TLR4-mediated NF-κB and MAPKs activation Food Sci Tech-Brazil. 2022 Mar;42:. WB Mouse 1:500

      Age-dependent accumulation of oligomeric SNCA/α-synuclein from impaired degradation in mutant LRRK2 knockin mouse model of Parkinson disease: role for therapeutic activation of chaperone-mediated autophagy (CMA). Autophagy Autophagy. 2020;16(2):347-370 WB,FC Mouse striatum , cortex,ventral midbrains

      TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-β1/Smads and activating P53 signaling pathways. Cell Death & Disease Cell Death Dis. 2020 Jan;11(1):1-15 WB Rat heart

      Bortezomib Inhibits Multiple Myeloma Cells by Transactivating ATF3 to Trigger miR-135a-5p- Dependent Apoptosis. Frontiers in Oncology Front Oncol. 2021; 11: 720261 WB Human 1:5000 RPMI-8226,U-266 cell

      KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma. Frontiers in Oncology Front Oncol. 2021; 11: 686678 WB Human 1 : 1000 Non-tumor renal tissue,ccRCC tumor tissue

      Treadmill Exercise Attenuates Cerebral Ischemia–Reperfusion Injury by Promoting Activation of M2 Microglia via Upregulation of Interleukin-4. Frontiers in Cardiovascular Medicine Front Cardiovasc Med. 2021 Oct;0:1282 WB Rat 1:5000 Penumbra

      The Role of Bone Morphogenetic Protein 9 in Nonalcoholic Fatty Liver Disease in Mice. Frontiers in Pharmacology Front Pharmacol. 2020; 11: 605967 WB Mouse Liver Hepatocytes

      Inhibitory role of ATF3 in gastric cancer progression through regulating cell EMT and stemness. Cancer Cell International Cancer Cell Int. 2021 Dec;21(1):1-10 WB Human 1:2000 SGC-7901 cell,MGC-803 cell

      Roles of miR-432 and circ_0000418 in mediating the anti-depressant action of ADAR1. Neurobiology of Stress 2021 Nov;15:100396 WB Human 1:5000 SH-SY5Y cell

      Prevotella copri ameliorates cholestasis and liver fibrosis in primary sclerosing cholangitis by enhancing the FXR signalling pathway. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE Bba-Mol Basis Dis. 2022 Mar;1868:166320 WB Mouse 1:10000 Liver ,SI tissue

      Exercise May Promote Skeletal Muscle Hypertrophy via Enhancing Leucine-Sensing: Preliminary Evidence. Frontiers in Physiology Front Physiol. 2021 Sep;0:1607 WB Rat 1:20000 Muscle

      MiR-581/SMAD7 Axis Contributes to Colorectal Cancer Metastasis: A Bioinformatic and Experimental Validation-Based Study. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2020 Sep 05 WB Human 1 : 5000 SW480 cell

      Comparative proteomics to reveal muscle-specific beef color stability of Holstein cattle during post-mortem storage. FOOD CHEMISTRY 2017 Mar 06 WB Bovine 1:20000 longissimuss lumborum (LL) , psoas major (PM))

      MiR‐496 promotes migration and epithelial‐mesenchymal transition by targeting RASSF6 in colorectal cancer. JOURNAL OF CELLULAR PHYSIOLOGY J Cell Physiol. 2020 Feb;235(2):1469-1479 WB Human 1:5000 LoVo cell, SW480 cell

      Shengjiang Xiexin Decoction Alters Pharmacokinetics of Irinotecan by Regulating Metabolic Enzymes and Transporters: A Multi-Target Therapy for Alleviating the Gastrointestinal Toxicity. Frontiers in Pharmacology Front Pharmacol. 2017 Oct;0:769 WB Rat 1:20000 livers , jejunum

      LNGFR targets the Wnt/β-catenin pathway and promotes the osteogenic differentiation in rat ectomesenchymal stem cells. Scientific Reports Sci Rep-Uk. 2017 Sep;7(1):1-14 WB Rat EMSCs

      Neuroprotective Effects of Qingnao Dripping Pills Against Cerebral Ischemia via Inhibiting NLRP3 Inflammasome Signaling Pathway: In Vivo and In Vitro. Frontiers in Pharmacology Front Pharmacol. 2020 Feb;0:65 WB Rat,Human left cerebral tissues SH-SY5Y cell

      Proto-Oncogene Serine/Threonine Kinase PIM3 Promotes Cell Migration via Modulating Rho GTPase Signaling. JOURNAL OF PROTEOME RESEARCH 2020 Jan 29 WB Human Huh7 cell,PLC cell,HCCLM3 cell, MHCC97L cell

      By Increasing the Expression and Activation of STAT3, Sustained C5a Stimulation Increases the Proliferation, Migration, and Invasion of RCC Cells and Promotes the Growth of Transgrafted Tumors. Cancer Management and Research Cancer Manag Res. 2021 Oct;13:7607-7621 WB Human 1:10000 786-O cell,ACHN cells

      Decreased level of miR-1301 promotes colorectal cancer progression via activation of STAT3 pathway. BIOLOGICAL CHEMISTRY Biol Chem. 2021 Jun;402(7):805-813 WB Human 1 : 5000 SW480 cell,LoVo cell

      Electroacupuncture Enhances Cognition by Promoting Brain Glucose Metabolism and Inhibiting Inflammation in the APP/PS1 Mouse Model of Alzheimer’s Disease: A Pilot Study. JOURNAL OF ALZHEIMERS DISEASE J Alzheimers Dis. 2020 Jan;77(1):387-400 WB Mouse 1 : 20000 frontal lobe

      Yizhiqingxin Formula Alleviates Cognitive Deficits and Enhances Autophagy via mTOR Signaling Pathway Modulation in Early Onset Alzheimer’s Disease Mice. Frontiers in Pharmacology Front Pharmacol. 2019 Sep;0:1041 WB Mouse hippocampus

      Stress aggravates and prolongs imiquimod‐induced psoriasis‐like epidermal hyperplasis and IL‐1β/IL‐23p40 production. JOURNAL OF LEUKOCYTE BIOLOGY J Leukocyte Biol. 2020 Jul;108(1):267-281 WB Mouse 1:40000 Skin

      Mesenchymal stem cell‑derived extracellular vesicles promote the in vitro proliferation and migration of breast cancer cells through the activation of the ERK pathway. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2019 May;54(5):1843-1852 WB Human 1:5000 hUC-MSC-EVs

      CD105 overexpression mediates drug-resistance in choriocarcinoma cells through BMP9/Smad pathway. Journal of Cancer J Cancer. 2020; 11(2): 272–283 WB Human 1:20000 JEG-3 cell

      Identification and Prognostic Value Exploration of Cyclophosphamide (Cytoxan)-Centered Chemotherapy Response-Associated Genes in Breast Cancer. DNA AND CELL BIOLOGY Dna Cell Biol. 2021 Nov;40(11):1356-1368 WB Human 1:5000 MDA-MB-231 cell,MDA-MB-435 cell,MCF-10 cell,MCF-7 cell

      Pressing Intervention Promotes the Skeletal Muscle Repair of Traumatic Myofascial Trigger Points in Rats. Journal of Pain Research J Pain Res. 2021; 14: 3267–3278 WB Rat MTrPs Tissue

      Noncanonical Roles of hα-syn (A53T) in the Pathogenesis of Parkinson’s Disease: Synaptic Pathology and Neuronal Aging. NEURAL PLASTICITY Neural Plast. 2020;2020:6283754 WB Mouse 1 : 5000 right midbrain,right striatum

      Association of RGS20 expression with the progression and prognosis of renal cell carcinoma. Oncology Letters Oncol Lett. 2021 Sep;22(3):1-11 WB Human 1:1000 HK-2, 786-O, A-498, SN12-PM6 , Caki-1

      Improvement in cerebral ischemia–reperfusion injury through the TLR4/NF-κB pathway after Kudiezi injection in rats. LIFE SCIENCES 2017 Oct 28 WB Rat 1:2000 ischemic ,control cortex

      Effect of RSK4 on Biological Characteristics of Gastric Cancer. Cancer Management and Research Cancer Manag Res. 2020; 12: 611–619 WB Human MGC-803 cell, HGC-27 cell

      miR-26 suppresses renal cell cancer via down-regulating coronin-3. MOLECULAR AND CELLULAR BIOCHEMISTRY 2019 Oct 08 WB Human 786-O cell,A-498 cell

      Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome. Frontiers in Psychiatry Front Psychiatry. 2020 Oct;0:1136 WB Mouse 1:5000 Brain , uterine

      Celastrol slows the progression of early diabetic nephropathy in rats via the PI3K/AKT pathway. BMC Complementary and Alternative Medicine Bmc Complem Altern M. 2020 Dec;20(1):1-14 WB Rat 1:20000 Renal

      Caveolin-3 promotes glycometabolism, growth and proliferation in muscle cells. PLoS One Plos One. 2017 Dec;12(12):e0189004 WB Mouse 1:500 C2C12 cell

      Traditional Chinese Medicine Shenmayizhi Decoction Ameliorates Memory and Cognitive Impairment Induced by Multiple Cerebral Infarctions. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2021;2021:6648455 WB Rat Brain

      miR‑128‑3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D. Oncology Letters Oncol Lett. 2021 Feb;21(2):1-1 WB Human 1 : 5000 hFOB1.19s, Saos-2s, U2OSs, MG-63s ,143B cell

      miR‑142‑5p promotes renal cell tumorigenesis by targeting TFAP2B. Oncology Letters Oncol Lett. 2020 Dec;20(6):1-1 WB Human 1 : 5000 A-498 cell,786-O cells,HK-2 cell

      Contribution of GABAergic modulation in DRGs to electroacupuncture analgesia in incisional neck pain rats. Journal of Pain Research J Pain Res. 2019; 12: 405–416 WB Rat 1:20000 dorsal root ganglion (DRG)

      The caveolin‐3 P104L mutation in LGMD‐1C patients inhibits non‐insulin‐stimulated glucose metabolism and growth but promotes myocyte proliferation. CELL BIOLOGY INTERNATIONAL 2019 May 13 WB Mouse 1:500 C2C12 cell

      Chronic heat stress increases insulin-like growth factor-1(IGF-1) but does not affect IGF-binding proteins in growing pigs. JOURNAL OF THERMAL BIOLOGY 2018 Aug 18 WB Pig 1: 20000 Liver

      WD-3 inhibits the proliferation of breast cancer cells by regulating the glycolytic pathway. Bosnian Journal of Basic Medical Sciences Bosnian J Basic Med. 2020 May; 20(2): 226–235 WB Human BT-549 cell, MDA-MB-231 cells,MCF-7 cell

      Acupuncture Needling, Electroacupuncture, and Fire Needling Improve Imiquimod-Induced Psoriasis-Like Skin Lesions through Reducing Local Inflammatory Responses. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2019;2019:4706865 WB Mouse 1:20000 skin

      Paeonol inhibits the development of 1‑chloro‑2,4‑dinitrobenzene‑induced atopic dermatitis via mast and T cells in BALB/c mice. Molecular Medicine Reports 2019 Apr 01 WB Mouse 1:20000 skin P815 cell

      Inhibition of microRNA‑155 ameliorates cardiac fibrosis in the process of angiotensin II‑induced cardiac remodeling. Molecular Medicine Reports Mol Med Rep. 2017 Nov;16(5):7287-7296 WB Mouse 1:1000 heart tissue cardiac fibroblasts

      Activation of TLR signalling regulates microwave radiation‐mediated impairment of spermatogenesis in rat testis. ANDROLOGIA Andrologia. 2018 Feb;50(1):e12828 WB Rat 1:20000 testis tissue

      Wei, Yuzhen, et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.

      Suppressive effect of β, β-dimethylacryloyl alkannin on activated dendritic cells in psoriasis by the TLR7/8 pathway." International immunopharmacology 40 (2016): 410-418.

      Jing, Shen, et al. "Imaging Potential Evaluation of Fab Derived from the Anti-EGFRvIII Monoclonal Antibody 4G1." Radiation research (2018).

      Chen, Luchao, et al. "Effects of purified Omphalia lapidescens protein on metastasis, cell cycle, apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.

      Jia, Yan-Fei, et al. "Expression of kisspeptin-GnRH system is down-regulated in hypothalamic arcuate nucleus of male rats with high-fat-diet." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10.5 (2017): 6099-6107.

      Niu, Yan-Long, et al. "Long-term strength training causes a downregulation of nephrin in the slit diaphragm of kidneys in rats." Int J Clin Exp Med 11.7 (2018): 6965-6972.

      谢万生, et al. "3 种 MAPK 抑制因子对亚砷酸钠活化 LX-2 细胞 TGF-β1 和 α-SMA 的影响." 中华地方病杂志 38.2: 96-100.

      Shi, Yanan, et al. "Salvianolic acid B ameliorates hepatocyte lipid droplet accumulation via stimulation of autophagy." (2020).

      Bai, Peide, et al. "miR-489-3p inhibits prostate cancer progression by targeting DLX1." Cancer management and research 12 (2020): 2719.

      武强, et al. "黑枸杞花青素通过 Ⅲ PI3K 通路调控小鼠巨噬细胞 RAW264. 7 自噬." 家畜生态学报 42.5: 33-37.

      Antidepressant and anxiolytic effects of the proprietary Chinese medicine Shexiang Baoxin pill in mice with chronic unpredictable mild stress. JOURNAL OF FOOD AND DRUG ANALYSIS 2018 Aug 24 WB Mouse 1:5000 hippocampus ,cerebral cortex

      The spatiotemporal expression and mineralization regulation of p75 neurotrophin receptor in the early tooth development. CELL PROLIFERATION 2018 Oct 25 WB,IHC Rat 1:2000 embryonic maxillofacial ectomesenchymal stem cells (EMSCs)

      Design, synthesis and biological evaluation of coumarin derivatives as potential BRD4 inhibitors BIOORGANIC CHEMISTRY Shi-Wu Chen WB Human

      RILP inhibits proliferation, migration, and invasion of PC3 prostate cancer cells ACTA HISTOCHEMICA Guoxiang Wang WB Human

      Aerobic Exercise Prevents Chronic Inflammation and Insulin Resistance in Skeletal Muscle of High-Fat Diet Mice Nutrients Yue Zhou WB Mouse

      Dietary fish oil improves autistic behaviors and gut homeostasis by altering the gut microbial composition in a mouse model of fragile X syndrome BRAIN BEHAVIOR AND IMMUNITY Yue Li WB Mouse colon

      Loss of Tumor Suppressor C9orf9 Promotes Metastasis in Colorectal Cancer Biomolecules Jin Yang WB Human SW480 cell,LoVo cell

      Immunoproteasome subunit β5i promotes perifascicular muscle atrophy in dermatomyositis by upregulating RIG-I RMD Open Qinglin Peng WB Human muscle

      Ezrin regulates the progression of NSCLC by YAP and PD-L1 Clinical & Translational Oncology Xu, Yirong WB Mouse,Human xenograft H520 cell, H1299 cell,A549 cell

      Akkermansia muciniphila-Nlrp3 is involved in the neuroprotection of phosphoglycerate mutase 5 deficiency in traumatic brain injury mice. Quanhua Xu WB Mouse 1:5000 cerebral cortical tissue

      MAOA suppresses the growth of gastric cancer by interacting with NDRG1 and regulating the Warburg effect through the PI3K/AKT/mTOR pathway. Li Jun WB Human 1:5000 BGC-823 cell,HGC-27 cell

      Network pharmacology and molecular docking prediction, combined with experimental validation to explore the potential mechanism of Qishen Yiqi pills against HF-related cognitive dysfunction. Hong-Cai Shang WB Rat hippocampus

      Taurine Protects against the Fatty Liver Hemorrhagic Syndrome in Laying Hens through the Regulation of Mitochondrial Homeostasis. Jiancheng Yang WB Human 1:5000 LO2 cell

      Upregulation of the CD155-CD226 Axis Is Associated With Muscle Inflammation and Disease Severity in Idiopathic Inflammatory Myopathies. Qinglin Peng WB Human 1:10000 muscle tissues

      MiR-3976 regulates HCT-8 cell apoptosis and parasite burden by targeting BCL2A1 in response to Cryptosporidium parvum infection. Rongjun Wang WB Human 1:2000 HCT-8 cell

      ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH Pengming Sun WB Human 1:2000 KLE cell,HEC-1A cell

      NLRP3-inflammasome inhibition by MCC950 attenuates cardiac and pulmonary artery remodelling in heart failure with preserved ejection fraction. LIFE SCIENCES Xiaocheng Cheng WB Mouse 1:5000 heart tissue

      Hydroxy-α-sanshool has anti-inflammatory and antioxidant effects and affects the NF-κB/STAT3 pathway via TRPV1 in acne. Journal of Functional Foods Xian Jiang WB Human 1:5000 HaCat cell, THP-1 cell

      Yueliang Yin Ameliorates Endometrial Receptivity in Mice with Embryo Implantation Failure by Reducing Pyroptosis and Activating BDNF/TrkB Pathway. MOLECULAR NUTRITION & FOOD RESEARCH Ting Wang WB Mouse uterus

      HMGB1 Mediates Inflammation-Induced DMT1 Increase and Dopaminergic Neurodegeneration in the Early Stage of Parkinsonism. MOLECULAR NEUROBIOLOGY Ke Ya WB rAT 1:5000 substantia nigra (SN)

      Cell Death & Disease Pengxing He WB Mouse,Human A549 cell-Xenograft A549 cell,H1975 cell,H1299 cell,H460 cell,H1650 cell,PC9 cell

      EUROPEAN JOURNAL OF PHARMACOLOGY Guo Ju Dong WB Rat heart tissue

      JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY Du-xiao Yang WB Rat spinal cord PC12 cell

      INTERNATIONAL JOURNAL OF MORPHOLOGY Lianbo Zhang WB Human 1:5000 Keloid fibroblasts (KFs),normal skin fibroblasts (NFs)

      INFLAMMATORY BOWEL DISEASES Li Tian WB Mouse,Human intestinal tissue,rectum tissue HT29 cell

      • Products Images
      • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD105(ABT-CD105)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: MCF7 Predicted band size: 38kDa Observed band size: 38kDa
      • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GAPDH(PTR2304)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: MCF7 Lane 3: THP-1 Lane 4: Ramos Predicted band size: 38kDa Observed band size: 38kDa
      • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GAPDH(PTR2304)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: C2C12 Lane 3: C2C12 Lane 4: K562 Lane 5: Raji Lane 6: 293F Lane 7: B16F10 Lane 8: Du145 Predicted band size: 38kDa Observed band size: 38kDa
      • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH(PTR2304) antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: MDCK Predicted band size: 38kDa Observed band size: 38kDa
      • Various whole cell lysates of K562,Mouse colon(10ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody. Lane 1: K562 Lane 2: Mouse colon Predicted band size: 38kDa Observed band size: 38kDa
      • Cheng, Xiaocheng, et al. "TNAP is a novel regulator of cardiac fibrosis after myocardial infarction by mediating TGF-β/Smads and ERK1/2 signaling pathways." EBioMedicine 67 (2021): 103370.
      • Wang, Yingying, et al. "p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathway." Cell proliferation 53.4 (2020): e12800.
      • Wei, Yuzhen, et al. "CD4+ CD25+ GARP+ regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy." Immunology 151.3 (2017): 291-303.
      • Zhang, Chengke, et al. "Induction of size-dependent breakdown of blood-milk barrier in lactating mice by TiO2 nanoparticles." PloS one 10.4 (2015): e0122591.
      • Du, Shaobo, et al. "Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells against Ultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation." BioMed research international 2017 (2017).
      • Chen, Luchao, et al. "Effects of purified Omphalia lapidescens protein on metastasis, cell cycle, apoptosis and the JAK-STAT signaling pathway in SGC-7901 human gastric cells." Oncology letters 15.4 (2018): 4161-4170.
      • Western blot analysis of 1 HEK293 2 SW480 3 HEPG2 4 MCF-7 5 mouse brain 6 Rat brain 7 Hela 8 A549 lysates, primary antibody was diluted at 1:5000, 4° over night, secondary antibody(cat: RS23910 was diluted at 1:10000, 37° 1hour.
      • Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle (3), Sheep Muscle (4), and Mouse brain (5), diluted at 1:10000.
      • The picture was kindly provided by our customer
      • The picture was kindly provided by our customer
      • Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GAPDH (PTR2304) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1:ACHN Lane 2: Daudi Lane 3: H69AR Lane 4: HACAT Lane 5:HUVEC Lane 6: Hela Lane 7: Jurkat Lane 8: K562 Predicted band size: 37kDa Observed band size: 37kDa
      • Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GAPDH (PTR2304) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1:H69AR Lane 2: LN18 Lane 3: LnCap Lane 4: MCF7 Lane 5:MES-SA Lane 6: MG-63 Lane 7: MOLT-4 Lane 8: NIH-3T3 Predicted band size: 37kDa Observed band size: 37kDa
      • Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GAPDH (PTR2304) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1:PC-3 Lane 2: PC-12 Lane 3: U2OS Lane 4: U-87 MG Lane 5:Karpas-299 Lane 6: Rabbit heart Predicted band size: 37kDa Observed band size: 37kDa