SNRPN Polyclonal Antibody
- 货号：YT5457
- 应用：WB;IHC
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Small nuclear ribonucleoprotein-associated protein N
- 免疫原：
- The antiserum was produced against synthesized peptide derived from the Internal region of human SNRPN. AA range:21-70
- 特异性：
- SNRPN Polyclonal Antibody detects endogenous levels of SNRPN protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SNRPN;HCERN3;SMN;Small nuclear ribonucleoprotein-associated protein N;snRNP-N;Sm protein D;Sm-D;Sm protein N;Sm-N;SmN;Tissue-specific-splicing protein
- 背景：
- The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing, possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splici
- 功能：
- disease:Patients with the autoimmune disease systemic lupus erythematosus (SLE) have autoantibodies directed against some of the individual snRNP polypeptides. The most common autoantigen is called Sm. N bears Sm epitopes.,function:May be involved in tissue-specific alternative RNA processing events.,miscellaneous:Encoded on a bicistronic transcript that encode for two proteins, SNRPN and SNURF.,miscellaneous:Encoded on a bicistronic transcript that encode for two proteins, SNRPN and SNURF. In addition to the primary 1.6-kb bicistronic SNURF-SNRPN transcript, SNURF-only transcript is also detected.,similarity:Belongs to the snRNP SmB/SmN family.,similarity:Belongs to the SNURF family.,subunit:Interacts with TDRD3.,tissue specificity:Expressed in brain and lymphoblasts.,tissue specificity:Expressed in heart, skeletal muscle and lymphoblasts (at protein level). Expressed in brain, pancreas
- 组织表达：
- Expressed in brain and lymphoblasts.
- Western Blot analysis of HeLa cells using SNRPN Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysate from HeLa cells, using SNRPN Antibody.
- Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
