Synaptotagmin Polyclonal Antibody

    • 货号:YT4484
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Synaptotagmin 1/2
      • 简介:
      • >>Synaptic vesicle cycle
      • 基因名称:
      • SYT1/SYT2
      • 蛋白名称:
      • Synaptotagmin-1/2
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human Synaptotagmin. AA range:276-325
      • 特异性:
      • Synaptotagmin Polyclonal Antibody detects endogenous levels of Synaptotagmin protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • SYT1;SVP65;SYT;Synaptotagmin-1;Synaptotagmin I;SytI;p65;SYT2;Synaptotagmin-2;Synaptotagmin II;SytII
      • 实测条带:
      • 50kD
      • 背景:
      • The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],
      • 功能:
      • cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.,domain:The first C2 domain mediates Ca(2+)-dependent phospholipid binding.,domain:The second C2 domain mediates interaction with SV2A and STN2.,function:May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.,similarity:Belongs to the synaptotagmin family.,similarity:Contains 2 C2 domains.,subcellular location:Synaptic vesicles and chromaffin granules.,subunit:H
      • 细胞定位:
      • Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Single-pass membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass membrane protein . Cytoplasm .
      • 组织表达:
      • Expressed in melanocytes (PubMed:23999003).

      Exosomes derived from bone-marrow mesenchymal stem cells alleviate cognitive decline in AD-like mice by improving BDNF-related neuropathology J Neuroinflamm. 2022 Dec;19(1):1-20. WB Mouse 1:1000 hippocampus
      货号:YT4484

      Potential value of Interleukin-6 as a diagnostic biomarker in human MDD and the antidepressant effect of its receptor antagonist tocilizumab in lipopolysaccharide-challenged rats. INTERNATIONAL IMMUNOPHARMACOLOGY Jinfang Ge WB Rat 1:1000 hippocampus
      货号:YT4484

      Potential Role of Bmal1 in Lipopolysaccharide-Induced Depression-Like Behavior and its Associated Journal of Neuroimmune Pharmacology Xu Dan-Dan WB Rat,Mouse 1:1000 hippocampus,hypothalamus BV2 cell
      货号:YT4484

      • 产品图片
      • Western Blot analysis of various cells using Synaptotagmin Polyclonal Antibody diluted at 1:2000
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Synaptotagmin Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from 293 cells, treated with Forskolin 40nM 30', using Synaptotagmin Antibody. The lane on the right is blocked with the synthesized peptide.