Repetin Polyclonal Antibody
- 货号：YT4052
- 应用：WB;IHC
- 种属：Human;Rat;Mouse;
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human RPTN. AA range:648-697
- 特异性：
- Repetin Polyclonal Antibody detects endogenous levels of Repetin protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景：
- domain:Can be divided into a N-terminal domain with significant homology to S100-like calcium-binding proteins, a central domain containing a series of short tandem repeats, and two flanking segments with low homology to the consensus sequences of the central repeats.,function:Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Potential substrate of transglutaminase. Some arginines are probably converted to citrullines by peptidylarginine deimidase.,similarity:Belongs to the S100-fused protein family.,similarity:Contains 2 EF-hand domains.,tissue specificity:Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheat and in the filiform papilli of the tongue.,
- 功能：
- domain:Can be divided into a N-terminal domain with significant homology to S100-like calcium-binding proteins, a central domain containing a series of short tandem repeats, and two flanking segments with low homology to the consensus sequences of the central repeats.,function:Involved in the cornified cell envelope formation. Multifunctional epidermal matrix protein. Reversibly binds calcium.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Potential substrate of transglutaminase. Some arginines are probably converted to citrullines by peptidylarginine deimidase.,similarity:Belongs to the S100-fused protein family.,similarity:Contains 2 EF-hand domains.,tissue specificity:Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheat and in the filiform papilli of the tongue.,
- 细胞定位：
- Secreted, extracellular space, extracellular matrix.
- 组织表达：
- Expression is scattered in the normal epidermis but strong in the acrosyringium, the inner hair root sheath and in the filiform papilli of the tongue.
- Western Blot analysis of HeLa cells using Repetin Polyclonal Antibody
- Western blot analysis of RPTN Antibody. The lane on the right is blocked with the RPTN peptide.
- Western blot analysis of the lysates from HepG2 cells using RPTN antibody.
- Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
