Repac Polyclonal Antibody
- 货号:YT4051
- 应用:WB;IHC
- 种属:Human;Mouse;Rat
- 简介:
- >>Ras signaling pathway;>>Rap1 signaling pathway
- 蛋白名称:
- Rap guanine nucleotide exchange factor 5
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RAPGEF5. AA range:431-480
- 特异性:
- Repac Polyclonal Antibody detects endogenous levels of Repac protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RAPGEF5;GFR;KIAA0277;MRGEF;Rap guanine nucleotide exchange factor 5;Guanine nucleotide exchange factor for Rap1;M-Ras-regulated Rap GEF;MR-GEF;Related to Epac;Repac
- 背景:
- Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008],
- 功能:
- function:Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,tissue specificity:Widely expressed with highest levels in brain.,
- 组织表达:
- Widely expressed with highest levels in brain.
- Western blot analysis of lysates from RAW264.7 cells, using RAPGEF5 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from COLO205 cells using RAPGEF5 antibody.
- Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).