Rag A/B Polyclonal Antibody
- 货号:YT3990
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Autophagy - animal;>>mTOR signaling pathway;>>Shigellosis
- 蛋白名称:
- Ras-related GTP-binding protein A/B
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human RRAGA/B. AA range:264-313
- 特异性:
- Rag A/B Polyclonal Antibody detects endogenous levels of Rag A/B protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RRAGA;Ras-related GTP-binding protein A;Rag A;RagA;Adenovirus E3 14.7 kDa-interacting protein 1;FIP-1;RRAGB;Ras-related GTP-binding protein B;Rag B;RagB
- 背景:
- function:Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.,similarity:Belongs to the GTR/RAG GTP-binding protein family.,subcellular location:Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state. Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm especially near the nuclear membrane and in discrete foci on or near the plasma membrane.,subunit:Can occur as a homodimer, or form a heterodimer with RRAGC or RRAGD in a sequence-independent manner. Binds GTP. The GTP-bound form of RRAGA interacts with NOL8. Interacts with adenovirus E3 14.7 kDa protein.,tissue specificity:Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.,
- 功能:
- function:Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.,similarity:Belongs to the GTR/RAG GTP-binding protein family.,subcellular location:Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state. Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm especially near the nuclear membrane and in discrete foci on or near the plasma membrane.,subunit:Can occur as a homodimer, or form a heterodimer with RRAGC or RRAGD in a sequence-independent manner. Binds GTP. The GTP-bound form of RRAGA interacts with NOL8. Interacts wi
- 细胞定位:
- Cytoplasm . Nucleus . Lysosome . Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state (PubMed:8995684, PubMed:9394008). Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm especially near the nuclear membrane and in discrete foci on or near the plasma membrane (PubMed:8995684). .
- 组织表达:
- Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
- Western Blot analysis of various cells using Rag A/B Polyclonal Antibody diluted at 1:1000
- Western Blot analysis of HepG2 cells using Rag A/B Polyclonal Antibody diluted at 1:1000
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HepG2 cells, using RRAGA/B Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using RRAGA/B antibody.