Pim-2 Polyclonal Antibody
- 货号：YT3729
- 应用：IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Pathways in cancer;>>Acute myeloid leukemia
- 蛋白名称：
- Serine/threonine-protein kinase pim-2
- 免疫原：
- Synthesized peptide derived from the C-terminal region of human Pim-2.
- 特异性：
- Pim-2 Polyclonal Antibody detects endogenous levels of Pim-2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PIM2;Serine/threonine-protein kinase pim-2;Pim-2h
- 背景：
- This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.[provided by RefSeq, Nov 2009],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells.,
- 组织表达：
- Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
