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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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Nek5 Polyclonal Antibody
- 货号:YT3033
- 应用:WB;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Serine/threonine-protein kinase Nek5
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human NEK5. AA range:160-209
- 特异性:
- Nek5 Polyclonal Antibody detects endogenous levels of Nek5 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NEK5;Serine/threonine-protein kinase Nek5;Never in mitosis A-related kinase 5;NimA-related protein kinase 5
- 背景:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,similarity:Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.,similarity:Contains 1 protein kinase domain.,
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,similarity:Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.,similarity:Contains 1 protein kinase domain.,
- Western Blot analysis of various cells using Nek5 Polyclonal Antibody
- Western blot analysis of NEK5 Antibody. The lane on the right is blocked with the NEK5 peptide.