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- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
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Nek4 Polyclonal Antibody
- 货号:YT3032
- 应用:IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Serine/threonine-protein kinase Nek4
- 免疫原:
- Synthesized peptide derived from Nek4 . at AA range: 200-280
- 特异性:
- Nek4 Polyclonal Antibody detects endogenous levels of Nek4 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NEK4;STK2;Serine/threonine-protein kinase Nek4;Never in mitosis A-related kinase 4;NimA-related protein kinase 4;Serine/threonine-protein kinase 2;Serine/threonine-protein kinase NRK2
- 背景:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Seems to act exclusively upon threonine residues.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas.,
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Seems to act exclusively upon threonine residues.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas.,
- 细胞定位:
- Cell projection, cilium . Cytoplasm .
- 组织表达:
- Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, testis, retina, liver, kidney, lung and placenta. Present in most primary carcinomas.
- Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200