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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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Mos Polyclonal Antibody
- 货号:YT2818
- 应用:WB;ELISA;IHC
- 种属:Human;Rat;Mouse;
- 简介:
- >>Oocyte meiosis;>>Regulation of actin cytoskeleton;>>Progesterone-mediated oocyte maturation
- 蛋白名称:
- Proto-oncogene serine/threonine-protein kinase mos
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MOS. AA range:61-110
- 特异性:
- Mos Polyclonal Antibody detects endogenous levels of Mos protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MOS;Proto-oncogene serine/threonine-protein kinase mos;Oocyte maturation factor mos;Proto-oncogene c-Mos
- 背景:
- MOS is a serine/threonine kinase that activates the MAP kinase cascade through direct phosphorylation of the MAP kinase activator MEK (MAP2K1; MIM 176872) (Prasad et al., 2008 [PubMed 18246541]).[supplied by OMIM, Jul 2009],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed specifically in testis during spermatogenesis.,
- 组织表达:
- Expressed specifically in testis during spermatogenesis.
- Western Blot analysis of various cells using Mos Polyclonal Antibody
- Western blot analysis of lysates from 293 cells, using MOS Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).