E-Ras Polyclonal Antibody
- 货号:YT1607
- 应用:WB;IF;ELISA
- 种属:Human;Rat;Mouse;
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human ERAS. AA range:184-233
- 特异性:
- E-Ras Polyclonal Antibody detects endogenous levels of E-Ras protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ERAS;HRAS2;HRASP;GTPase ERas;E-Ras;Embryonic stem cell-expressed Ras
- 背景:
- This gene encodes a constitutively active member of the small GTPase Ras protein family. The encoded protein activates the phosphatidylinositol 3-kinase signal transduction pathway in undifferentiated stem cells, but is not expressed in differentiated cells. This gene may be involved in cancer and chemotherapy resistance. [provided by RefSeq, Dec 2012],
- 功能:
- enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).,function:Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.,similarity:Belongs to the small GTPase superfamily. Ras family.,subunit:Interacts with PIK3CD.,
- 细胞定位:
- Cell membrane ; Lipid-anchor ; Cytoplasmic side .
- Immunofluorescence analysis of A549 cells, using ERAS Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using ERAS Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using ERAS antibody.