Di-Ras1 Polyclonal Antibody
- 货号:YT1353
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- GTP-binding protein Di-Ras1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human DIRA1. AA range:149-198
- 特异性:
- Di-Ras1 Polyclonal Antibody detects endogenous levels of Di-Ras1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DIRAS1;GBTS1;RIG;GTP-binding protein Di-Ras1;Distinct subgroup of the Ras family member 1;Ras-related inhibitor of cell growth;Rig;Small GTP-binding tumor suppressor 1
- 背景:
- DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004],
- 功能:
- function:Displays low GTPase activity and exist predominantly in the GTP-bound form.,similarity:Belongs to the small GTPase superfamily. Di-Ras family.,tissue specificity:Highly expressed in heart and brain.,
- 细胞定位:
- Cell membrane ; Lipid-anchor ; Cytoplasmic side .
- 组织表达:
- Highly expressed in heart and brain.
- Western Blot analysis of various cells using Di-Ras1 Polyclonal Antibody
- Western blot analysis of lysates from HeLa and HepG2 cells, using DIRA1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).