Di-Ras1 Polyclonal Antibody

    • 货号:YT1353
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Rat;Mouse;
      • 靶点:
      • Di-Ras1
      • 基因名称:
      • DIRAS1
      • 蛋白名称:
      • GTP-binding protein Di-Ras1
      • Human Swiss Prot No:
      • O95057
      • Mouse Swiss Prot No:
      • Q91Z61
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human DIRA1. AA range:149-198
      • 特异性:
      • Di-Ras1 Polyclonal Antibody detects endogenous levels of Di-Ras1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • DIRAS1;GBTS1;RIG;GTP-binding protein Di-Ras1;Distinct subgroup of the Ras family member 1;Ras-related inhibitor of cell growth;Rig;Small GTP-binding tumor suppressor 1
      • 实测条带:
      • 22kD
      • 背景:
      • DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004],
      • 功能:
      • function:Displays low GTPase activity and exist predominantly in the GTP-bound form.,similarity:Belongs to the small GTPase superfamily. Di-Ras family.,tissue specificity:Highly expressed in heart and brain.,
      • 细胞定位:
      • Cell membrane ; Lipid-anchor ; Cytoplasmic side .
      • 组织表达:
      • Highly expressed in heart and brain.
      • 产品图片
      • Western Blot analysis of various cells using Di-Ras1 Polyclonal Antibody
      • Western blot analysis of lysates from HeLa and HepG2 cells, using DIRA1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).