PP2A-Cα (phospho Tyr307) Polyclonal Antibody

    • 货号:YP0889
    • 应用:WB;IP;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • PP2AA
      • 简介:
      • >>mRNA surveillance pathway;>>Sphingolipid signaling pathway;>>Oocyte meiosis;>>Autophagy - other;>>Autophagy - animal;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Adrenergic signaling in cardiomyocytes;>>TGF-beta signaling pathway;>>Hippo signaling pathway;>>Tight junction;>>Dopaminergic synapse;>>Long-term depression;>>Chagas disease;>>Hepatitis C;>>Human papillomavirus infection
      • 基因名称:
      • PPP2CA
      • 蛋白名称:
      • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
      • Human Gene Id:
      • 5515
      • Human Swiss Prot No:
      • P67775
      • Mouse Swiss Prot No:
      • P63330
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307. AA range:260-309
      • 特异性:
      • Phospho-PP2A-Cα (Y307) Polyclonal Antibody detects endogenous levels of PP2A-Cα protein only when phosphorylated at Y307.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • PPP2CA;Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;Replication protein C;RP-C
      • 实测条带:
      • 35kD
      • 背景:
      • This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008],
      • 功能:
      • catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,function:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.,PTM:Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation
      • 细胞定位:
      • Cytoplasm . Nucleus . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle pole . In prometaphase cells, but not in anaphase cells, localizes at centromeres (PubMed:16541025). During mitosis, also found at spindle poles (PubMed:16541025). Centromeric localization requires the presence of SGO2 (By similarity). .
      • 组织表达:
      • Fibroblast,Liver,Lung,Placenta,Testis,Uterus,

      Microcystin-Leucine-Arginine Induces Tau Pathology Through Bα Degradation via Protein Phosphatase 2A Demethylation and Associated Glycogen Synthase Kinase-3β Phosphorylation. TOXICOLOGICAL SCIENCES 2018 Apr 01 IP Human SH-SY5Y cell
      货号:YP0889

      Zhang, Yali, et al. "Microcystin-LR induces tau pathology through Bα degradation via PP2A demethylation and associated GSK-3β phosphorylation." Toxicological Sciences(2017).
      货号:YP0889

      • 产品图片
      • Immunofluorescence analysis of Hela cell. 1,PP2A-Cα (phospho Tyr307) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). HSP70 Monoclonal Antibody(3G10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western blot analysis of SKOV3 using p-PP2A-Cα (Y307) antibody. Antibody was diluted at 1:1000
      • Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.
      • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The lane on the right is blocked with the phospho peptide.