VE-Cadherin (phospho Tyr731) Polyclonal Antibody

    • 货号:YP0808
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • VE-Cadherin
      • 简介:
      • >>Cell adhesion molecules;>>Leukocyte transendothelial migration;>>Fluid shear stress and atherosclerosis
      • 基因名称:
      • CDH5
      • 蛋白名称:
      • Cadherin-5
      • Human Gene Id:
      • 1003
      • Human Swiss Prot No:
      • P33151
      • Mouse Swiss Prot No:
      • P55284
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human VE-Cadherin around the phosphorylation site of Tyr731. AA range:697-746
      • 特异性:
      • Phospho-VE-Cadherin (Y731) Polyclonal Antibody detects endogenous levels of VE-Cadherin protein only when phosphorylated at Y731.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CDH5;Cadherin-5;7B4 antigen;Vascular endothelial cadherin;VE-cadherin;CD antigen CD144
      • 实测条带:
      • 130kD
      • 背景:
      • This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015],
      • 功能:
      • function:Cadherins are calcium dependent cell adhesion proteins.,function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.,similarity:Contains 5 cadherin domains.,subcellular location:Found at cell-cell boundaries and probably at cell-matrix boundaries.,tissue specificity:Endothelial tissues and brain.,
      • 细胞定位:
      • Cell junction . Cell membrane ; Single-pass type I membrane protein . Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions. .
      • 组织表达:
      • Endothelial tissues and brain.

      Tumor necrosis factor ligand-related molecule 1A maintains blood–retinal barrier via modulating SHP-1-Src-VE-cadherin signaling in diabetic retinopathy. FASEB JOURNAL Faseb J. 2021 Nov;35(11):e22008 WB Rat,Human 1:1000 HRMECs,RRMECs
      货号:YP0808

      • 产品图片
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using VE-Cadherin (Phospho-Tyr731) Antibody
      • Immunohistochemistry analysis of paraffin-embedded human brain, using VE-Cadherin (Phospho-Tyr731) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using VE-Cadherin (Phospho-Tyr731) Antibody. The lane on the right is blocked with the phospho peptide.