EDEM3 Polyclonal Antibody
- 货号:YN0703
- 应用:WB;ELISA
- 种属:Human;Mouse
- 简介:
- >>Protein processing in endoplasmic reticulum
- 蛋白名称:
- ER degradation-enhancing alpha-mannosidase-like 3
- 免疫原:
- Synthesized peptide derived from part region of human protein
- 特异性:
- EDEM3 Polyclonal Antibody detects endogenous levels of protein.
- 组成:
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000 ELISA 1:5000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed 16431915]).[supplied by OMIM, Mar 2008],
- 功能:
- domain:Contains a protease-associated domain (PA) of unknown function.,function:Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes. This process depends on mannose-trimming from the N-glycans. Seems to have alpha 1,2-mannosidase activity.,PTM:N-glycosylated.,similarity:Belongs to the glycosyl hydrolase 47 family.,similarity:Contains 1 PA (protease associated) domain.,
- 细胞定位:
- Endoplasmic reticulum lumen .
- 组织表达:
- Brain,Liver,Lung,Placenta,Testis,Whole embr
- Western Blot analysis of Hela lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000