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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PSA (ABT-PSA) Mouse mAb
- 货号:YM6091
- 应用:IHC;WB;ELISA
- 种属:Human
- 简介:
- >>Pathways in cancer;>>Prostate cancer
- 蛋白名称:
- Prostate-specific antigen (PSA) (EC 3.4.21.77) (Gamma-seminoprotein) (Seminin) (Kallikrein-3) (P-30 antigen) (Semenogelase)
- 免疫原:
- Synthesized peptide derived from human Prostate-Specific Antigen(PSA) AA range: 100-200
- 特异性:
- This antibody detects endogenous levels of human Prostate-Specific Antigen(PSA). Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin se
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Mouse, Monoclonal/IgG1, Kappa
- 稀释:
- IHC 1:200-400, WB 1:200-1000, ELISA 1:5000-20000
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:Preferential cleavage: -Tyr-|-Xaa-.,function:Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.,online information:Prostate-specific antigen entry,similarity:Belongs to the peptidase S1 family.,similarity:Belongs to the peptidase S1 family. Kallikrein subfamily.,similarity:Contains 1 peptidase S1 domain.,
- 组织表达:
- Leukocyte,PNS,Prostate,
- LnCap whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-PSA(ABT-PSA)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.
Lane 1: LnCap
Predicted band size: 28kDa
Observed band size: 28kDa
- Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PSA (ABT-PSA)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.
Lane 1: LnCap
Predicted band size: 28kDa
Observed band size: 35kDa
- Human prostate tissue was stained with Anti-Prostate-Specific Antigen (ABT-PSA) Antibody
- Human prostatic adenocarcinoma tissue was stained with Anti-Prostate-Specific Antigen (ABT-PSA) Antibody